Kim In-Yong, Choi Hyungsoo, Kim Kyekyoon Kevin
a Department of Electrical and Computer Engineering , University of Illinois , Urbana , IL , USA.
b Micro and Nanotechnology Laboratory , University of Illinois , Urbana , IL , USA.
J Microencapsul. 2017 Feb;34(1):57-62. doi: 10.1080/02652048.2017.1284275. Epub 2017 Feb 9.
In this study, we investigated the effect of intracapsular environment on the survival of anchorage-dependent cells (ADCs) encapsulated in alginate microcapsules with three different core structures, i.e. liquid, semi-liquid and microsphere-encapsulating semi-liquid core, using NIH 3T3 fibroblasts as an ADC model. For the latter, we fabricated poly (ɛ-caprolactone) microspheres and co-encapsulated them with the cells, to establish cell-substrate interactions in the capsule. The fibroblast cells co-encapsulated with the microspheres exhibited higher survival and growth than those without. This study provides a "proof of concept" for employing microspheres as a cell-friendly surface to establish intracapsular cell-substrate interactions thus prolonging the survival of encapsulated therapeutic ADCs.
在本研究中，我们以NIH 3T3成纤维细胞作为锚定依赖性细胞（ADC）模型，研究了囊内环境对封装在具有三种不同核心结构（即液体、半液体和微球封装半液体核心）的藻酸盐微胶囊中的ADC存活的影响。对于后者，我们制备了聚（ε-己内酯）微球并将它们与细胞共同封装，以在胶囊中建立细胞-基质相互作用。与微球共同封装的成纤维细胞比未与微球共同封装的细胞表现出更高的存活率和生长率。本研究为采用微球作为细胞友好表面以建立囊内细胞-基质相互作用从而延长封装的治疗性ADC的存活提供了“概念验证”。
