Dong Fang, Zhou Ying, Zeng Lanting, Watanabe Naoharu, Su Xinguo, Yang Ziyin
Guangdong Food and Drug Vocational College, Longdongbei Road 321, Tianhe District, Guangzhou 510520, China.
Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.
Molecules. 2017 Jan 13;22(1):131. doi: 10.3390/molecules22010131.
1-Phenylethanol (1PE) can be used as a fragrance in food flavoring and cosmetic industries and as an intermediate in the pharmaceutical industry. 1PE can be synthesized from acetophenone, and the cost of 1PE is higher than the cost of acetophenone. Therefore, it is important to establish an effective and low-cost approach for producing 1PE. Our previous studies found that tea () flowers, which are an abundant and waste resource, contained enzymes that could transform acetophenone to 1PE. In the present study, we extracted crude enzymes from tea flowers and optimized the production conditions of 1PE using response surface methodology. The optimized conditions were an extraction pH of 7.0, a reaction pH of 5.3, a reaction temperature of 55 °C, a reaction time of 100 min, a coenzyme NADPH concentration of 3.75 μmol/mL in the reaction assay, and a substrate acetophenone concentration of 1.25 μmol/mL in the reaction assay. The results provide essential information for future industrial 1PE production using plant-derived enzymes.
1-苯乙醇（1PE）可作为香料用于食品调味和化妆品行业，也可作为制药行业的中间体。1PE可由苯乙酮合成，且1PE的成本高于苯乙酮。因此，建立一种有效且低成本的1PE生产方法很重要。我们之前的研究发现，茶（）花作为一种丰富的废弃资源，含有可将苯乙酮转化为1PE的酶。在本研究中，我们从茶花中提取了粗酶，并使用响应面法优化了1PE的生产条件。优化后的条件为：提取pH值为7.0，反应pH值为5.3，反应温度为55℃，反应时间为100分钟，反应测定中辅酶NADPH浓度为3.75μmol/mL，反应测定中底物苯乙酮浓度为1.25μmol/mL。这些结果为未来利用植物源酶进行工业1PE生产提供了重要信息。
