Liang Tingming, Yu Jiafeng, Liu Chang, Guo Li
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210023, P.R. China.
Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics, Institute of Biophysics, Dezhou University, Dezhou, Shandong 253023, P.R. China.
Mol Med Rep. 2017 Mar;15(3):1071-1078. doi: 10.3892/mmr.2017.6117. Epub 2017 Jan 13.
Multiple microRNA (miRNA) variants, known as isomiRs, are extensively distributed in miRNA loci and predominantly derive from the alternative cleavage of Drosha/Dicer and 3'addition events. The present study aimed to investigate the expression patterns of multiple isomiRs in typical miRNA and Dicer‑independent miRNA loci by conducting evolutionary and expression analysis using public datasets. Although different miRNA maturation processes exist, multiple isomiRs can be detected by similar expression distributions. However, isomiR expression in Dicer‑independent miRNA loci tends to be at a moderate level, particularly for random distribution in the ends that are split by Dicer in the typical miRNA loci. Compared with the mature miRNA locus (dominant miRNA locus), the non‑dominant miRNA locus indicates an expression distribution similar to that of the Dicer‑independent miRNA locus. These results increase the understanding of multiple isomiRs in the progression of diseases.
多种微小RNA（miRNA）变体，即异源miRNA（isomiRs），广泛分布于miRNA基因座中，主要来源于Drosha/Dicer的选择性切割和3'加尾事件。本研究旨在通过使用公共数据集进行进化和表达分析，研究典型miRNA和不依赖Dicer的miRNA基因座中多种异源miRNA的表达模式。尽管存在不同的miRNA成熟过程，但通过相似的表达分布可以检测到多种异源miRNA。然而，不依赖Dicer的miRNA基因座中的异源miRNA表达往往处于中等水平，特别是在典型miRNA基因座中由Dicer切割的末端的随机分布情况。与成熟miRNA基因座（主要miRNA基因座）相比，非主要miRNA基因座的表达分布与不依赖Dicer的miRNA基因座相似。这些结果加深了对疾病进展过程中多种异源miRNA的理解。
