Jin Lu, Li Yifan, He Tao, Hu Jia, Liu Jiaju, Chen Mingwei, Zhang Zeng, Gui Yaoting, Mao Xiangming, Yang Shangqi, Lai Yongqing
Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
Anhui Medical University, Hefei, Anhui 230032, P.R. China.
Mol Med Rep. 2017 Mar;15(3):1379-1386. doi: 10.3892/mmr.2017.6121. Epub 2017 Jan 16.
miRNAs have been reported to be involved in multiple cellular processes and the tumorigenesis of various cancers. miR‑15a‑5p (also termed miR‑15a) has previously been determined to be upregulated in renal cell carcinoma (RCC) by microarray profile. However, the expression and function of miR‑15a‑5p in RCC remain to be validated. In the present study, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to detect the expression levels of miR‑15a‑5p in RCC tissues and cells. The expression level of miR‑15a‑5p was upregulated or downregulated by transfecting synthesized miR‑15a‑5p mimics or inhibitors. The MTT assay, CCK‑8 assay, Transwell assay, wound healing assay, Hoechest 33342 staining and flow cytometry were conducted to investigate the role of miR‑15a‑5p in RCC. The results of the RT‑qPCR demonstrated that miR‑15a‑5p was upregulated in RCC tissues and ACHN, 786‑O and 769P RCC cells compared with paired normal tissues and HEK‑293T cells. miR‑15a‑5p was observed to be associated with RCC cell proliferation, migration, invasion and apoptosis. The results demonstrated that miR‑15a‑5p may be important as a tumor promoter in RCC. To the best of our knowledge, the present study is the first to describe miR‑15a‑5p as a tumor promoter in RCC. Further research will be performed to investigate the underlying signaling pathway of miR‑15a‑5p and the potential role of miR‑15a‑5p as a biomarker for early detection, prognosis prediction and a therapeutic target of RCC.
据报道，微小RNA（miRNA）参与多种细胞过程以及各种癌症的肿瘤发生。之前通过微阵列分析确定miR-15a-5p（也称为miR-15a）在肾细胞癌（RCC）中上调。然而，miR-15a-5p在RCC中的表达和功能仍有待验证。在本研究中，进行逆转录定量聚合酶链反应（RT-qPCR）以检测RCC组织和细胞中miR-15a-5p的表达水平。通过转染合成的miR-15a-5p模拟物或抑制剂来上调或下调miR-15a-5p的表达水平。进行MTT法、CCK-8法、Transwell法、伤口愈合试验、Hoechest 33342染色和流式细胞术以研究miR-15a-5p在RCC中的作用。RT-qPCR结果表明，与配对的正常组织和HEK-293T细胞相比，miR-15a-5p在RCC组织以及ACHN、786-O和769P RCC细胞中上调。观察到miR-15a-5p与RCC细胞增殖、迁移、侵袭和凋亡相关。结果表明，miR-15a-5p可能作为RCC中的肿瘤促进因子发挥重要作用。据我们所知，本研究首次将miR-15a-5p描述为RCC中的肿瘤促进因子。将进行进一步研究以探讨miR-15a-5p的潜在信号通路以及miR-15a-5p作为RCC早期检测、预后预测生物标志物和治疗靶点的潜在作用。
