Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
Mol Med Rep. 2017 Dec;16(6):9157-9164. doi: 10.3892/mmr.2017.7705. Epub 2017 Oct 4.
Micro (mi)RNAs are involved in multiple cellular processes, and alterations in miRNA expression have been demonstrated to lead to tumorigenesis. Previous microarray analysis revealed that miRNA (miR)‑24 was downregulated in renal cell carcinoma (RCC). Additionally, miR‑24 has been identified as an oncogene and tumor suppressor in various cancers. The present study assessed the expression levels of two stem‑loops of miR‑24, miR‑24‑1 and miR‑24‑2, in RCC tissues and paired healthy tissues by reverse transcription‑quantitative polymerase chain reaction. The results revealed that miR‑24‑2 was upregulated in RCC tissues and ACHN, 786‑O and 769P cell lines compared with healthy tissues and HEK‑293T cells, respectively, whereas miR‑24‑1 was almost absent in RCC and healthy kidney tissues. To investigate the role of miR‑24‑2 in RCC, a synthesized miR‑24‑2 mimic, negative control (NC), inhibitor or inhibitor NC was transfected into 786‑O and ACHN RCC cells, and cell proliferation, mobility and apoptosis assays were performed. The results of the present study revealed that miR‑24‑2 was associated with cell proliferation, migration, invasion and apoptosis, thus demonstrating that miR‑24‑2 may serve a role as an oncogene in RCC. Further studies are required to investigate the signaling pathways of miR‑24‑2, and the potential of miR‑24‑2 as a therapeutic target or biomarker for the early detection of RCC.
微小 RNA（miRNA）参与多种细胞过程，并且 miRNA 表达的改变已被证明可导致肿瘤发生。先前的微阵列分析显示，miRNA（miR）-24 在肾细胞癌（RCC）中下调。此外，miR-24 已被鉴定为多种癌症中的癌基因和肿瘤抑制因子。本研究通过逆转录-定量聚合酶链反应评估了 RCC 组织和配对的健康组织中 miR-24 的两个茎环 miR-24-1 和 miR-24-2 的表达水平。结果显示，miR-24-2 在 RCC 组织和 ACHN、786-O 和 769P 细胞系中上调，与健康组织和 HEK-293T 细胞相比，而 miR-24-1 在 RCC 和健康肾组织中几乎不存在。为了研究 miR-24-2 在 RCC 中的作用，将合成的 miR-24-2 模拟物、阴性对照（NC）、抑制剂或抑制剂 NC 转染到 786-O 和 ACHN RCC 细胞中，并进行细胞增殖、迁移和凋亡检测。本研究结果表明，miR-24-2 与细胞增殖、迁移、侵袭和凋亡有关，表明 miR-24-2 可能在 RCC 中作为癌基因发挥作用。需要进一步研究 miR-24-2 的信号通路，以及 miR-24-2 作为 RCC 早期检测的治疗靶点或生物标志物的潜力。
