Laboratoire d'Electrochimie Moléculaire, UMR 7591 CNRS, Université Paris Diderot, Sorbonne Paris Cité, 15 rue Jean-Antoine de Baïf, F-75205, Paris Cedex 13, France.
Centre de Recherche Paul-Pascal, UPR 8641 CNRS, Université de Bordeaux, 115 avenue Schweitzer, 33600, Pessac, France.
Small. 2017 Apr;13(13). doi: 10.1002/smll.201603163. Epub 2017 Jan 18.
Organizing active enzyme molecules on nanometer-sized scaffolds is a promising strategy for designing highly efficient supported catalytic systems for biosynthetic and sensing applications. This is achieved by designing model nanoscale enzymatic platforms followed by thorough analysis of the catalytic activity. Herein, the virus fd bacteriophage is considered as an enzyme nanocarrier to study the scaffolding effects on enzymatic activity. Nanoarrays of randomly oriented, or directionally patterned, fd bacteriophage virus are functionalized with the enzyme glucose oxidase (GOx), using an immunological assembly strategy, directly on a gold electrode support. The scaffolding process on the virus capsid is monitored in situ by AFM (atomic force microscopy) imaging, while cyclic voltammetry is used to interrogate the catalytic activity of the resulting functional GOx-fd nanoarrays. Kinetic analysis reveals the ability to modulate the activity of GOx via nanocarrier patterning. The results evidence, for the first time, enhancement of the enzymatic activity due to scaffolding on a filamentous viral particle.
在纳米级支架上组织活性酶分子是设计用于生物合成和传感应用的高效负载催化系统的有前途的策略。通过设计模型纳米尺度酶平台并对催化活性进行彻底分析，可以实现这一目标。在这里，病毒 fd 噬菌体被视为酶纳米载体，以研究支架效应对酶活性的影响。使用免疫组装策略，将随机定向或定向图案化的 fd 噬菌体病毒纳米阵列功能化为葡萄糖氧化酶 (GOx)，直接在金电极载体上。通过原子力显微镜 (AFM) 成像原位监测病毒衣壳上的支架过程，同时使用循环伏安法检测所得功能 GOx-fd 纳米阵列的催化活性。动力学分析表明可以通过纳米载体图案化来调节 GOx 的活性。这些结果首次证明，由于丝状病毒颗粒上的支架作用，酶活性得到增强。
