Chen Xiao P, Chao Yong S, Chen Wen Z, Dong Jing Y
a College of Biological and Environmental Engineering, Zhejiang University of Technology , Hangzhou , China.
b School of Medicine and Life Sciences, Zhejiang University City College , Hangzhou , China.
J Environ Sci Health B. 2017 Feb;52(2):77-83. doi: 10.1080/03601234.2016.1239973. Epub 2016 Oct 24.
Chlorpyrifos (CPF) is a widely used organophosphorus pesticide with developmental neurotoxicity such as morphogenesis toxicity. In the present study, we assessed the effects of prenatal CPF exposure on systemic parameters and cytoarchitecture of medial prefrontal cortex (mPFC) in adulthood. Gestational dams were exposed to 5mg/kg/d of CPF during gestational days 13-17, while body weight, organ coefficient, and neuron and glia counts of offspring were determined on postnatal day 60. Our results showed that CPF treatment induced little or no effects on body weight and organ coefficients. There were also no significant pathological changes in mPFC. However, neuron and glia count analysis showed that CPF treatment reduced neuron and glia counts in anterior cingulate, prelimbic, and infralimbic areas of mPFC. The CPF react pattern was similar in both sexes, and there was no statistical difference in most of the sub-regions. Thus, our results revealed an embryonic origin brain deficit induced by gestational mother pesticide exposure.
毒死蜱（CPF）是一种广泛使用的有机磷农药，具有发育神经毒性，如形态发生毒性。在本研究中，我们评估了产前暴露于毒死蜱对成年期内侧前额叶皮质（mPFC）的全身参数和细胞结构的影响。妊娠母鼠在妊娠第13至17天暴露于5mg/kg/d的毒死蜱，而在出生后第60天测定后代的体重、器官系数以及神经元和神经胶质细胞数量。我们的结果表明，毒死蜱处理对体重和器官系数几乎没有影响。mPFC也没有明显的病理变化。然而，神经元和神经胶质细胞数量分析表明，毒死蜱处理减少了mPFC前扣带回、前额叶和边缘下区域的神经元和神经胶质细胞数量。毒死蜱的反应模式在两性中相似，并且在大多数子区域中没有统计学差异。因此，我们的结果揭示了妊娠母体农药暴露导致的胚胎源性脑缺陷。
