Spencer J, Isaacson P G, Walker-Smith J A, MacDonald T T
Department of Histopathology, University College London, England.
J Pediatr Gastroenterol Nutr. 1989 Aug;9(2):173-7. doi: 10.1097/00005176-198908000-00007.
Intraepithelial lymphocytes (IEL) are present in fetal human small intestine from 14 weeks' gestation independent of exogenous dietary or microbial antigens. We have now studied the heterogeneity of IEL in 18-22-week-old human fetal intestine and in postnatal small intestine by single and sequential immunoenzyme histochemistry. In normal children and adults, there were 6-27 CD3+ IEL per 100 epithelial cells, whereas in fetal gut there were 3-5 CD3+ IEL per 100 epithelial cells. Postnatal intestine contained a population of CD3-,7+, non-T cell IEL (7-25% of total CD7+). These cells were absent from fetal IEL but were occasionally seen in the fetal lamina propria. About 6% of CD3+ postnatal IEL were CD4-,8-. In contrast in the fetus, 35-70% of CD3+ IEL were subset negative. Since CD3 and CD7 are always co-expressed on fetal IEL, 28-58% of fetal IEL were also CD7+,4-,8-. Only about 20% of the CD3+ IEL expressed the gamma delta chains of the T cell antigen receptor. We conclude from these studies that CD3+,4-,8- T cells migrate to the epithelium in the absence of exogenous antigen and that there is a population of CD3-,7+ non-T cells in postnatal gut which is absent in fetal gut.
上皮内淋巴细胞（IEL）在妊娠14周起就存在于人类胎儿小肠中，与外源性饮食或微生物抗原无关。我们现在通过单步和连续免疫酶组织化学研究了18 - 22周龄人类胎儿小肠和出生后小肠中IEL的异质性。在正常儿童和成人中，每100个上皮细胞中有6 - 27个CD3 + IEL，而在胎儿肠道中，每100个上皮细胞中有3 - 5个CD3 + IEL。出生后的小肠含有一群CD3 - 、7 + 非T细胞IEL（占总CD7 + 的7 - 25%）。这些细胞在胎儿IEL中不存在，但偶尔可见于胎儿固有层。出生后约6%的CD3 + IEL为CD4 - 、8 - 。相比之下，在胎儿中，35 - 70%的CD3 + IEL为亚群阴性。由于CD3和CD7在胎儿IEL上总是共表达，28 - 58%的胎儿IEL也为CD7 + 、4 - 、8 - 。只有约20%的CD3 + IEL表达T细胞抗原受体的γδ链。我们从这些研究中得出结论，CD3 + 、4 - 、8 - T细胞在没有外源性抗原的情况下迁移到上皮，并且出生后肠道中有一群CD3 - 、7 + 非T细胞，而胎儿肠道中不存在。
