Tan Wenlong, Song Zi-Zheng, Xu Qunfang, Qu Xinyan, Li Zhen, Wang Yu, Yu Qun, Wang Shengqi
Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, China (mainland).
II Department of Medical Oncology, PLA General Hospital, Beijing , China (mainland).
Med Sci Monit. 2017 Jan 18;23:309-314. doi: 10.12659/msm.898369.
BACKGROUND Long non-coding RNA SPRY4 intronic transcript 1 (lncRNA SPRY4-IT1) has been reported to be associated with the progression of several cancers, but its expression level in colorectal cancer (CRC) has rarely been reported. The purpose of this study was to estimate the clinical significance of SPRY4-IT1 in CRC. MATERIAL AND METHODS The relative expression levels of SPRY4-IT1 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) in diseased tissues and the adjacent normal tissues of 106 CRC patients. Chi-square method was used to evaluate the association between SPRY4-IT1 expression and the clinical features. Additionally, we assessed the overall survival at different expression levels of SPRY4-IT1 using Kaplan-Meier method. The prognostic significance of SPRY4-IT1 was estimated by Cox regression analysis. RESULTS Up-regulated level of SPRY4-IT1 was detected in pathologic tissues of CRC patients compared with adjacent normal tissues (P=0.000). The relative expression of SPRY4-IT1 was associated with the tumor size, the depth of invasion, lymph node invasion, distant invasion, and tumor stage (P<0.05). Patients with high expression of SPRY4-IT1 had poor overall survival compared with those with high level (39.3 vs. 49.3 months, log-rank test, P=0.016). Cox regression analysis showed that SPRY4-IT1 could act as an independent prognostic factor in CRC (HR=2.341, 95% CI=1.136-4.826, P=0.021). CONCLUSIONS SPRY4-IT1 might be associated with tumorigenesis and progression of CRC, and it may be a promising biomarker for prognosis in patients with CRC.
背景 长链非编码RNA SPRY4内含子转录本1（lncRNA SPRY4-IT1）已被报道与多种癌症的进展相关，但其在结直肠癌（CRC）中的表达水平鲜有报道。本研究旨在评估SPRY4-IT1在CRC中的临床意义。
材料与方法 采用定量实时聚合酶链反应（qRT-PCR）检测106例CRC患者病变组织及癌旁正常组织中SPRY4-IT1的相对表达水平。采用卡方检验评估SPRY4-IT1表达与临床特征之间的关联。此外，我们使用Kaplan-Meier法评估不同SPRY4-IT1表达水平下的总生存期。通过Cox回归分析评估SPRY4-IT1的预后意义。
结果 与癌旁正常组织相比，CRC患者病理组织中检测到SPRY4-IT1表达上调（P = 0.000）。SPRY4-IT1的相对表达与肿瘤大小、浸润深度、淋巴结转移、远处转移及肿瘤分期相关（P < 0.05）。与低表达患者相比，SPRY4-IT1高表达患者的总生存期较差（39.3个月对49.3个月，对数秩检验，P = 0.016）。Cox回归分析表明，SPRY4-IT1可作为CRC的独立预后因素（HR = 2.341，95%CI = 1.136 - 4.826，P = 0.021）。
结论 SPRY4-IT1可能与CRC的发生和进展相关，它可能是CRC患者预后的一个有前景的生物标志物。
