Zheng Q, Wu F, Dai W-Y, Zheng D-C, Zheng C, Ye H, Zhou B, Chen J-J, Chen P
Department of General Surgery, Ningbo No. 2 Hospital, Ningbo, 315000, China.
Clin Transl Oncol. 2015 Aug;17(8):640-6. doi: 10.1007/s12094-015-1290-2. Epub 2015 Apr 24.
Long noncoding RNAs (lncRNAs) have been shown to regulate tumor biology and might be used for cancer diagnosis, prognosis and potential therapeutic targets. Although up-regulation of lncRNA UCA1 (urothelial carcinoma-associated 1) in several cancers has been found, its role in gastric cancer remains elusive. The aim of this study was to detect the expression of lncRNA UCA1 in gastric cancer and its clinical association. The expression of UCA1 was detected in 112 pairs of tumorous and adjacent normal tissues from patients with gastric cancer, as well as in four gastric cancer cell lines and a human normal gastric epithelium cell line using RT-qPCR. Results showed that UCA1 expression was remarkably increased in gastric cancer tissues and cell lines compared with that in the normal control. Clinicopathologic analysis revealed that high UCA1 expression correlated with worse differentiation, tumor size, invasion depth and TNM stage in gastric cancer. Kaplan-Meier analysis showed that increased UCA1 expression contributed to poor overall survival (p = 0.017) and disease-free survival (p = 0.024) of patients. A multivariate survival analysis also indicated that UCA1 could be an independent prognostic marker. The levels of UCA1 in gastric juice from gastric patients were significantly higher than those from normal subjects (p = 0.016). Moreover, validation analysis showed that UCA1 levels were robust in differentiating gastric cancer patients from control subjects [area under the curve (AUC) = 0.721; 95 % confidence interval (CI) = 0.655-0.788, p < 0.01]. These results suggested that UCA1 might serve as a promising biomarker for early detection and prognosis prediction of gastric cancer.
长链非编码RNA（lncRNAs）已被证明可调节肿瘤生物学特性，并可能用于癌症的诊断、预后评估及潜在治疗靶点。尽管已发现在几种癌症中lncRNA UCA1（尿路上皮癌相关1）上调，但其在胃癌中的作用仍不清楚。本研究旨在检测lncRNA UCA1在胃癌中的表达及其临床相关性。采用RT-qPCR检测了112对胃癌患者的肿瘤组织和癌旁正常组织，以及四种胃癌细胞系和一种人正常胃上皮细胞系中UCA1的表达。结果显示，与正常对照相比，UCA1在胃癌组织和细胞系中的表达显著增加。临床病理分析显示，胃癌中UCA1高表达与较差的分化程度、肿瘤大小、浸润深度和TNM分期相关。Kaplan-Meier分析表明，UCA1表达增加导致患者总生存期（p = 0.017）和无病生存期（p = 0.024）较差。多因素生存分析也表明UCA1可能是一个独立的预后标志物。胃癌患者胃液中UCA1水平显著高于正常受试者（p = 0.016）。此外，验证分析表明，UCA1水平在区分胃癌患者和对照受试者方面具有稳健性[曲线下面积（AUC）= 0.721；95%置信区间（CI）= 0.655 - 0.788，p < 0.01]。这些结果表明，UCA1可能是胃癌早期检测和预后预测的一个有前景的生物标志物。
