美国牛犊血清的病毒组
Virome of US bovine calf serum.
作者信息
Sadeghi Mohammadreza, Kapusinszky Beatrix, Yugo Danielle M, Phan Tung Gia, Deng Xutao, Kanevsky Isis, Opriessnig Tanja, Woolums Amelia R, Hurley David J, Meng Xiang-Jin, Delwart Eric
机构信息
Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA; Department of Virology, University of Helsinki, Finland.
Blood Systems Research Institute, San Francisco, CA, USA; Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.
出版信息
Biologicals. 2017 Mar;46:64-67. doi: 10.1016/j.biologicals.2016.12.009. Epub 2017 Jan 15.
Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses. This study further characterizes the diversity of viruses in calf serum with the potential to infect fetuses and through fetal bovine serum contaminate cell cultures.
我们使用病毒宏基因组学分析了从美国715头小牛采集的4组牛血清样本。分别在3组和4组样本中检测到两种细小病毒，即牛细小病毒2型（BPV2）和一种以前未鉴定的细小病毒，命名为牛沙病毒（BosaV），并生成了它们的完整编码序列。基于NS1蛋白的同一性，牛沙病毒被鉴定为细小病毒属中的一个新物种。同时还检测到少量与有蹄类动物四细小病毒2型、牛丙型肝炎病毒和几种乳头瘤病毒匹配的读数。本研究进一步描述了小牛血清中可能感染胎儿并通过胎牛血清污染细胞培养物的病毒多样性。
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