Fujino H, Hidaka M, Goya S
Yakugaku Zasshi. 1989 Aug;109(8):606-10. doi: 10.1248/yakushi1947.109.8_606.
Anthracene-1-carbonyl azide was found to be a sensitive fluorescent derivatization reagent for primary and secondary alcohols for high-performance liquid chromatography. Reaction conditions were investigated for cholestanol. The reagent reacts with alcohols in benzene in the presence of 4-dimethylaminopyridine to give the corresponding fluorescent urethanes at 100 degrees C for 50 min. The detection limit for cholestanol was 250 fmol for an injection volume of 10 microliters. Tertiary alcohols and phenols do not produce fluorescent derivatives under these conditions.
蒽-1-羰基叠氮化物被发现是一种用于高效液相色谱分析伯醇和仲醇的灵敏荧光衍生试剂。研究了胆甾烷醇的反应条件。该试剂在4-二甲基氨基吡啶存在下于苯中与醇反应，在100℃下反应50分钟，生成相应的荧光氨基甲酸酯。当进样体积为10微升时，胆甾烷醇的检测限为250飞摩尔。叔醇和酚在这些条件下不会产生荧光衍生物。
