Fujino H, Koya S
Department of Pharmacy, Kitakyushu Tuyazaki Hospital, Fukuoka, Japan.
Yakugaku Zasshi. 1994 Oct;114(10):794-8. doi: 10.1248/yakushi1947.114.10_794.
Ethyl 1-(difluoro-1,3,5-triazinyl)-2-methylindolizine-3-carboxylate was found to be a selective fluorescence derivatization reagent for primary and secondary amines. The reagent reacted with amines in aqueous acetonitrile in the presence of sodium hydroxide to give the corresponding fluorescent products, which could be separated on a TSK gel ODS-80TM reversed-phase column with aqueous methanol as eluent. 2-Phenethylamine and spermidine were used to investigate the derivatization conditions. The detection limits (signal-to-noise ratio = 3) of each compound were 3 and 2 pmol per 10 microliters injection volume, respectively. Alcohols, thiols and aromatic amines did not give any fluorescent products under these derivatization conditions.
发现1-（二氟-1,3,5-三嗪基）-2-甲基中氮茚-3-羧酸乙酯是一种用于伯胺和仲胺的选择性荧光衍生试剂。该试剂在氢氧化钠存在下于乙腈水溶液中与胺反应，生成相应的荧光产物，这些产物可用甲醇水溶液作为洗脱剂在TSK凝胶ODS - 80TM反相柱上进行分离。使用2-苯乙胺和亚精胺研究衍生化条件。每次进样体积为10微升时，每种化合物的检测限（信噪比 = 3）分别为3皮摩尔和2皮摩尔。在这些衍生化条件下，醇类、硫醇类和芳香胺类未产生任何荧光产物。
