Characterization of 16 microsatellite markers for the clade of (Adoxaceae).

PREMISE OF THE STUDY

Microsatellite loci were isolated from four species of (Adoxaceae) to study population structure and assess species boundaries among morphologically similar South American species of the clade.

METHODS AND RESULTS

Using a microsatellite-enriched library and mining next-generation sequence data, 16 microsatellites were developed. Each locus was tested on two populations of and one population of . For nuclear loci, one to 13 alleles were recovered, expected heterozygosity ranged from 0 to 0.8975, Simpson diversity index ranged from 0.0167 to 1.000, and Shannon diversity index ranged from 0 to 2.3670 in a given population. For the mitochondrial locus, three to six alleles were recovered and unbiased haploid diversity values ranged from 0.756 to 0.853 in a given population.

CONCLUSIONS

The 16 microsatellite loci developed for the clade (, Adoxaceae) will inform investigations of population structure and species boundaries within this group.