Hamm Trinity P, Nowicki Marcin, Boggess Sarah L, Klingeman William E, Hadziabdic Denita, Huff Matthew L, Staton Margaret E, Trigiano Robert N
Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996-4560, USA.
Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996-4561, USA.
Plants (Basel). 2021 Mar 5;10(3):487. doi: 10.3390/plants10030487.
The genus is of particular interest to horticulturalists, phylogeneticists, and biogeographers. Despite its popularity, there are few existing molecular markers to investigate genetic diversity in this large genus, which includes over 160 species. There are also few polymorphic molecular tools that can delineate closely related species within the genus. , a member of the subclade and one of the centers of diversity for , was selected for DNA sequencing and development of genomic simple sequence repeats (gSSRs). In this study, 15 polymorphic gSSRs were developed and characterized for a collection of 19 samples. Number of alleles per locus ranged from two- to- eight and nine loci had four or more alleles. Observed heterozygosity ranged from 0 to 0.84 and expected heterozygosity ranged from 0.10 to 0.80 for the 15 loci. Shannon diversity index values across these loci ranged from 0.21 to 1.62. The markers developed in this study add to the existing molecular toolkit for the genus and will be used in future studies investigating cross-transferability, genetic variation, and species and cultivar delimitation in the genus and closely allied genera in the Adoxaceae and Caprifoliaceae.
该属对于园艺学家、系统发育学家和生物地理学家具有特别的吸引力。尽管它很受欢迎，但在这个包含160多个物种的大属中，用于研究遗传多样性的现有分子标记很少。也几乎没有能区分该属内亲缘关系密切的物种的多态性分子工具。 作为亚分支的成员以及 的多样性中心之一，被选来进行DNA测序和基因组简单序列重复（gSSRs）的开发。在本研究中，针对19个 样本的集合开发并表征了15个多态性gSSRs。每个位点的等位基因数从2到8不等，9个位点有4个或更多等位基因。15个位点的观察杂合度范围为0至0.84，预期杂合度范围为0.10至0.80。这些位点的香农多样性指数值范围为0.21至1.62。本研究中开发的标记物增加了该属现有的分子工具集，并将用于未来研究五福花科和忍冬科中该属及亲缘关系密切的属的交叉转移性、遗传变异以及物种和品种的界定。
