Molina Da Silva G P, Tanaka O M, Campos Navarro D F, Repeke C E, Garlet G P, Guariza-Filho O, Trevilatto P C
School of Life Sciences Curitiba, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil.
School of Dentistry of Bauru, Department of Biological Sciences, FOB/USP, Bauru, Brazil.
Orthod Craniofac Res. 2017 Feb;20(1):30-34. doi: 10.1111/ocr.12137.
The aim of this study was to analyze MMP-1 transcript levels in periodontal tissues of rats that underwent orthodontic treatment using potassium diclofenac and dexamethasone at different stages of tooth movement.
The sample comprised of ninety male Wistar rats.
A closed nickel-titanium coil spring was used to apply a force of 50 cN to move the maxillary right first molars mesially. One group received daily doses of 0.9% saline solution, the second group received daily doses of 5 mg/kg potassium diclofenac, and the third group received daily doses of 0.5 mg/kg dexamethasone. Tooth movement was observed on days 0, 1, 3, 7, and 14. MMP-1 transcript levels were evaluated by real-time polymerase chain reaction and the results were compared between groups by three-way ANOVA, with a significance level of 0.05.
Transcript levels increased in groups that received the coil spring treatment on all days of the experiment. MMP-1 expression was found to be decreased in groups treated with potassium diclofenac and dexamethasone compared to that in the control group, on days 1, 3, 5, and 7.
The application of orthodontic forces significantly increased MMP-1 transcript levels. The use of anti-inflammatory drugs may have an inhibitory effect on MMP-1 expression.
本研究旨在分析在牙齿移动不同阶段使用双氯芬酸钾和地塞米松进行正畸治疗的大鼠牙周组织中基质金属蛋白酶-1（MMP-1）转录水平。
样本包括90只雄性Wistar大鼠。
使用闭合镍钛螺旋弹簧施加50 cN的力使上颌右侧第一磨牙近中移动。一组每日给予0.9%生理盐水，第二组每日给予5 mg/kg双氯芬酸钾，第三组每日给予0.5 mg/kg地塞米松。在第0、1、3、7和14天观察牙齿移动情况。通过实时聚合酶链反应评估MMP-1转录水平，并通过三因素方差分析比较各组结果，显著性水平为0.05。
在实验的所有天数中，接受螺旋弹簧治疗的组转录水平均升高。在第1、3、5和7天，发现双氯芬酸钾和地塞米松治疗组的MMP-1表达与对照组相比有所降低。
正畸力的施加显著提高了MMP-1转录水平。使用抗炎药物可能对MMP-1表达有抑制作用。
