Pontifical Catholic University of Paraná, Curitiba, Brazil.
Anat Rec (Hoboken). 2010 Feb;293(2):195-9. doi: 10.1002/ar.21035.
Potassium diclofenac is a potent nonsteroidal anti-inflammatory drug (NSAID) and COX isoforms (COX-1 and COX-2) inhibitor. Quantitative analysis of birefringence with polarized light microscopy is a useful method to investigate the macromolecular orientation and organization of collagen fibers in connective tissues. The aim of this research was to analyze the collagen structure and maturation in bone formed after potassium diclofenac administration, during first molar orthodontic movement. Sixty Wistar rats were divided in two equal groups (N = 30): control (C) and potassium diclofenac (PD). The animals in Group C received 0.9% saline solution and the PD group received potassium diclofenac Cataflam (5 mg/kg). Animals were sacrificed 3, 7, or 14 days after a NiTi unilateral closed-coil spring was stretched between the upper right first molar and the incisors. The first molar area was fixed, decalcified, and histologically processed using picrosirius pigment. The collagen birefringence of bone turnover was analyzed by phase retardation. Two-way ANOVA and Tukey's test showed that optical retardation was influenced by time and treatment. There was increase in the collagen organization over time. On the third day, the C group showed better collagen organization than the PD group. Potassium diclofenac interfered in collagen maturation, reducing fibril organization in the initial phase of orthodontic movement.
双氯芬酸钾是一种强效的非甾体抗炎药（NSAID）和环氧化酶同工酶（COX-1 和 COX-2）抑制剂。偏光显微镜下的双折射定量分析是一种研究结缔组织中胶原蛋白纤维的宏观取向和组织的有用方法。本研究旨在分析正畸牙移动过程中，双氯芬酸钾给药后形成的牙齿矿化骨中的胶原结构和成熟情况。60 只 Wistar 大鼠随机分为两组（N = 30）：对照组（C）和双氯芬酸钾组（PD）。C 组给予 0.9%生理盐水，PD 组给予双氯芬酸钾（5mg/kg）。在 NiTi 单侧闭合线圈弹簧在上颌右侧第一磨牙和切牙之间拉伸 3、7 或 14 天后，处死动物。第一磨牙区固定、脱钙，并用苦味酸天狼猩红染色进行组织学处理。通过相差延迟分析骨转换的胶原双折射。双因素方差分析和 Tukey 检验显示，光延迟受时间和处理的影响。胶原组织随时间增加而增加。第 3 天，C 组的胶原组织比 PD 组更好。双氯芬酸钾干扰胶原成熟，减少正畸运动初始阶段的纤维组织排列。
