Dental Materials, Pontifical Catholic University of Rio Grande do Sul, Ipiranga Av. 6681, Patenon, Porto Alegre-RS, Brazil 90619-900.
Microsc Res Tech. 2011 Aug;74(8):709-13. doi: 10.1002/jemt.20947. Epub 2010 Dec 3.
Bone formation is essential to orthodontic tooth movement and bone is formed by collagen. To analyze the collagen maturation process on bone matrix neoformed under nonsteroidal and steroidal treatment during orthodontic tooth movement by polarized microscopy, male Wistar rats (n = 90) were randomly divided into three groups (n = 30): C (control), NSAID (potassium diclofenac) and SAID (disodic phosphate dexamethasone). The animals of the C group received 0.9% saline solution; NSAID group received 5 mg/kg potassium diclofenac (CATAFLAM®); and SAID group received 2 mg/kg phosphate dissodic dexamethasone (DEXANIL®). Animals were sacrificed 3, 7 or 14 days after the placement of orthodontic appliances and the upper first molars were processed histologically and stained with picrosirius. Bone formation was evaluated under polarized light microscopy and 4.5 Image Pro-Plus® software calculated the percentage of immature/mature collagen present in the groups. On the third days after force application, SAID and NSAID groups showed greater proportion of immature collagen than C group. On the seventh and fourteenth days, there was a lower proportion of mature collagen only in the SAID group (P < 0.001). These data demonstrate that dexamethasone delays the collagen maturation process in established bone matrix.
骨形成对于正畸牙齿移动至关重要，而骨是由胶原蛋白形成的。为了通过偏光显微镜分析在正畸牙齿移动期间非甾体和甾体治疗下新形成的骨基质中的胶原蛋白成熟过程，雄性 Wistar 大鼠（n = 90）被随机分为三组（n = 30）：C（对照）、NSAID（双氯芬酸钾）和 SAID（磷酸二氢钠地塞米松）。C 组的动物接受 0.9%生理盐水；NSAID 组给予 5 mg/kg 双氯芬酸钾（CATAFLAM®）；SAID 组给予 2 mg/kg 磷酸二氢钠地塞米松（DEXANIL®）。在放置正畸矫治器后 3、7 或 14 天处死动物，并对上颌第一磨牙进行组织学处理和苦味酸天狼星红染色。在偏光显微镜下评估骨形成，并使用 4.5 Image Pro-Plus®软件计算各组中存在的不成熟/成熟胶原蛋白的百分比。在力施加后的第三天，SAID 和 NSAID 组的不成熟胶原蛋白比例高于 C 组。在第 7 天和第 14 天，仅在 SAID 组中成熟胶原蛋白的比例较低（P < 0.001）。这些数据表明地塞米松延迟了已建立的骨基质中的胶原蛋白成熟过程。
