Navarrete-Bolaños J L, Téllez-Martínez M G, Miranda-López R, Jiménez-Islas H
a Departamento de Ingeniería Bioquímica , Instituto Tecnológico de Celaya , Celaya , Gto. , México.
Prep Biochem Biotechnol. 2017 Jul 3;47(6):578-588. doi: 10.1080/10826068.2017.1280825. Epub 2017 Jan 19.
For any fermentation process, the production cost depends on several factors, such as the genetics of the microorganism, the process condition, and the culture medium composition. In this work, a guideline for the design of cost-efficient culture media using a sequential approach based on response surface methodology is described. The procedure was applied to analyze and optimize a culture medium of registered trademark and a base culture medium obtained as a result of the screening analysis from different culture media used to grow the same strain according to the literature. During the experiments, the procedure quantitatively identified an appropriate array of micronutrients to obtain a significant yield and find a minimum number of culture medium ingredients without limiting the process efficiency. The resultant culture medium showed an efficiency that compares favorably with the registered trademark medium at a 95% lower cost as well as reduced the number of ingredients in the base culture medium by 60% without limiting the process efficiency. These results demonstrated that, aside from satisfying the qualitative requirements, an optimum quantity of each constituent is needed to obtain a cost-effective culture medium. Study process variables for optimized culture medium and scaling-up production for the optimal values are desirable.
对于任何发酵过程，生产成本取决于几个因素，如微生物的遗传学、工艺条件和培养基组成。在这项工作中，描述了一种基于响应面法的顺序方法来设计具有成本效益的培养基的指南。该程序被应用于分析和优化一种注册商标培养基以及一种基础培养基，该基础培养基是根据文献从用于培养同一菌株的不同培养基的筛选分析中获得的。在实验过程中，该程序定量地确定了一系列合适的微量营养素，以获得显著的产量，并在不限制工艺效率的情况下找到最少数量的培养基成分。所得培养基显示出的效率与注册商标培养基相当，成本降低了95%，同时在不限制工艺效率的情况下，基础培养基中的成分数量减少了60%。这些结果表明，除了满足质量要求外，还需要每种成分的最佳数量来获得具有成本效益的培养基。研究优化培养基的过程变量并按比例放大生产以获得最佳值是可取的。
