Kasiotis Konstantinos M, Anastasiadou Pelagia, Papadopoulos Antonis, Machera Kyriaki
Benaki Phytopathological Institute, Department of Pesticides Control and Phytopharmacy, Laboratory of Pesticides' Toxicology, Kifissia, Athens, Greece.
Benaki Phytopathological Institute, Department of Phytopathology, Laboratory of Non-Parasitic Diseases, Kifissia, Athens, Greece.
PLoS One. 2017 Jan 19;12(1):e0170077. doi: 10.1371/journal.pone.0170077. eCollection 2017.
Propolis is a bee product that has been extensively used in alternative medicine and recently has gained interest on a global scale as an essential ingredient of healthy foods and cosmetics. Propolis is also considered to improve human health and to prevent diseases such as inflammation, heart disease, diabetes and even cancer. However, the claimed effects are anticipated to be correlated to its chemical composition. Since propolis is a natural product, its composition is consequently expected to be variable depending on the local flora alignment. In this work, we present the development of a novel HPLC-PDA-ESI/MS targeted method, used to identify and quantify 59 phenolic compounds in Greek propolis hydroalcoholic extracts. Amongst them, nine phenolic compounds are herein reported for the first time in Greek propolis. Alongside GC-MS complementary analysis was employed, unveiling eight additional newly reported compounds. The antioxidant activity study of the propolis samples verified the potential of these extracts to effectively scavenge radicals, with the extract of Imathia region exhibiting comparable antioxidant activity to that of quercetin.
蜂胶是一种蜂产品，已在替代医学中广泛使用，最近作为健康食品和化妆品的重要成分在全球范围内受到关注。蜂胶还被认为可以改善人类健康并预防诸如炎症、心脏病、糖尿病甚至癌症等疾病。然而，所宣称的效果预计与其化学成分相关。由于蜂胶是一种天然产物，因此其成分预计会因当地植物群落的不同而有所变化。在这项工作中，我们展示了一种新型的HPLC-PDA-ESI/MS靶向方法的开发，该方法用于鉴定和定量希腊蜂胶水醇提取物中的59种酚类化合物。其中，9种酚类化合物首次在希腊蜂胶中被报道。同时采用了GC-MS互补分析，揭示了另外8种新报道的化合物。蜂胶样品的抗氧化活性研究证实了这些提取物有效清除自由基的潜力，伊马夏地区的提取物表现出与槲皮素相当的抗氧化活性。
