Wang Minjiao, Li Hongliang, Si Jiawen, Dai Jiewen, Shi Jun, Wang Xudong, Guo Lihe, Shen Guofang
Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Acta Biochim Biophys Sin (Shanghai). 2017 Mar 1;49(3):197-207. doi: 10.1093/abbs/gmw133.
Stem cells isolated from the amniotic fluid have been shown as a promising candidate for cell therapy and tissue engineering. However, the experimental and preclinical applications of amniotic fluid-derived stem cells (AFSCs) in the very field of maxillofacial bone tissue engineering are still limited. In this study, rat AFSCs were successfully harvested and characterized in vitro. The rat AFSCs showed typical fibroblastoid morphology, stable proliferation activity and multi-differentiation potential. Flow-cytometry analysis demonstrated that these cells were positive for CD29, CD44, and CD90, while negative for hematopoietic markers such as CD34 and CD45. The regenerative performance of AFSCs-premixed with platelet rich plasma (PRP) gel in restoration of alveolar bone defect was further investigated using a modified rat maxillary alveolar defect model. Micro-computer tomography and histological examination showed a superior regenerative capacity of AFSCs-premixed with PRP gel at both 4 and 8 weeks after operation comparing with control groups. Moreover, the implanted AFSCs can survive in the defect site and directly participate in the bone tissue regeneration. Taken together, these results indicated the feasibility of an AFSCs-based alveolar bone tissue engineering strategy for alveolar defect restoration.
从羊水中分离出的干细胞已被证明是细胞治疗和组织工程的一个有前景的候选者。然而，羊水来源的干细胞（AFSCs）在颌面骨组织工程领域的实验和临床前应用仍然有限。在本研究中，成功地收获了大鼠AFSCs并在体外进行了表征。大鼠AFSCs表现出典型的成纤维细胞样形态、稳定的增殖活性和多分化潜能。流式细胞术分析表明，这些细胞CD29、CD44和CD90呈阳性，而造血标志物如CD34和CD45呈阴性。使用改良的大鼠上颌牙槽骨缺损模型进一步研究了AFSCs与富血小板血浆（PRP）凝胶预混合在牙槽骨缺损修复中的再生性能。微型计算机断层扫描和组织学检查显示，与对照组相比，AFSCs与PRP凝胶预混合在术后4周和8周时均具有更强的再生能力。此外，植入的AFSCs能够在缺损部位存活并直接参与骨组织再生。综上所述，这些结果表明基于AFSCs的牙槽骨组织工程策略用于牙槽骨缺损修复的可行性。
