Wei Jianping, Nie Qingmei, Li Feng
Department of Emergency, Weifang People's Hospital, Weifang, Shandong 261000, P.R. China.
Department of Health Care, Weifang People's Hospital, Weifang, Shandong 261000, P.R. China.
Exp Ther Med. 2016 Dec;12(6):3694-3698. doi: 10.3892/etm.2016.3806. Epub 2016 Oct 14.
The aim of the present study was to examine the effect of lamotrigine and the expression of myeloid-related protein 8 (MRP8) and interleukin-7 (IL-7) in the treatment of focal cortical dysplasia with secondary intractable epilepsy. In this study, rats with focal cortical dysplasia with secondary intractable epilepsy (constructed by our laboratory) were selected and used for experimentation, 21-day Sprague-Dawley rats were randomly divided into the control group (38 rats), the observation group I (39 rats), and the observation group II (38 rats). Rats in the observation group I received daily intraperitoneal injection of 0.02 mg/kg lamotrigine, and those in the observation group II and the control group received daily intraperitoneal injection of 0.02 mg/kg normal saline. Expression quantities of MRP8 and IL-7 in the hippocampus sample tissues of mice in the control group, observation group I, and observation group II were measured via fluorescence quantitative polymerase chain reaction assay, western blot analysis, enzyme-linked immunosorbent assay, and immunohistochemistry 48 h later. and gene mRNA levels of the control group, the observation group I and the observation group II had no significant differences (P>0.05). The expression quantity on the protein level of MRP8 and IL-7 showed no significant differences (P>0.05) between the observation group I (7.91±1.3, 3.86±0.38) and the control group (7.52±1.03, 3.62±0.29). The expression quantity of MRP8 and IL-7 showed significant differences (P<0.05) between observation group II (27.47±1.13, 19.45±0.48) and observation group I (7.91±1.3, 3.86±0.38). It was found that MRP8 and IL-7 were focused on the nerve cell membrane of hippocampus of rats in the observation group by immunohistochemistry experiments. In conclusion, the results from the present study show that lamotrigine can be used to treat rats with focal cortical dysplasia with secondary intractable epilepsy by reducing the expression levels of MRP8 and IL-7 in the body, providing a new therapeutic target to the follow-up treatment of focal cortical dysplasia with secondary intractable disease.
本研究旨在探讨拉莫三嗪对继发性难治性癫痫局灶性皮质发育不良的治疗效果以及髓样相关蛋白8（MRP8）和白细胞介素-7（IL-7）的表达情况。本研究选取了由本实验室构建的继发性难治性癫痫局灶性皮质发育不良大鼠进行实验，将21日龄的斯普拉格-道利大鼠随机分为对照组（38只）、观察组I（39只）和观察组II（38只）。观察组I的大鼠每日腹腔注射0.02 mg/kg拉莫三嗪，观察组II和对照组的大鼠每日腹腔注射0.02 mg/kg生理盐水。48小时后，通过荧光定量聚合酶链反应检测、蛋白质印迹分析、酶联免疫吸附测定和免疫组织化学法检测对照组、观察组I和观察组II小鼠海马样本组织中MRP8和IL-7的表达量。对照组、观察组I和观察组II的 和 基因mRNA水平无显著差异（P>0.05）。观察组I（7.91±1.3, 3.86±0.38）和对照组（7.52±1.03, 3.62±0.29）之间MRP8和IL-7蛋白水平的表达量无显著差异（P>0.05）。观察组II（27.47±1.13, 19.45±0.48）和观察组I（7.91±1.3, 3.86±0.38）之间MRP8和IL-7的表达量有显著差异（P<0.05）。免疫组织化学实验发现，MRP8和IL-7聚焦于观察组大鼠海马的神经细胞膜上。总之，本研究结果表明，拉莫三嗪可通过降低体内MRP8和IL-7的表达水平来治疗继发性难治性癫痫局灶性皮质发育不良大鼠，为继发性难治性疾病局灶性皮质发育不良的后续治疗提供了新的治疗靶点。
