Lin Yingzi, Wu Qiang, Liu Xiang, Dong Sufang, Wu Lixian, Pei Hua, Xu Ke, Xia Qianfeng
Key Laboratory of Translational Medicine for Tropical Diseases, Ministry of Education, and Faculty of Tropical Medicine and Laboratory Medicine, Hainan Medical University, Haikou, Hainan 571199, P.R. China.
Biomed Rep. 2016 Dec;5(6):766-770. doi: 10.3892/br.2016.795. Epub 2016 Oct 27.
Sporadic cases of melioidosis have been reported in Hainan, China for decades; however, to the best of our knowledge, there are no accurate source-identification investigations confirming that melioidosis is endemic. Four indigenous melioidosis cases were identified, which prompted the performance of contact microbiologic and molecular techniques to evaluate endemicity. Environmental samples were collected from various locations surrounding each patient's residence. The samples were screened for () using Ashdown culture medium, and confirmed by polymerase chain reaction and 16S ribosomal DNA sequencing. Clinical and environmental isolates of were evaluated by multilocus sequence typing (MLST) and 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for evidence of homology between them. Analysis by MLST indicated that one environmental sample and one clinical colony were sequence type-46, as well as type (8, 3, 11, 9) by MLVA-4. The evidence indicates a likely geographical and epidemiological association. Taken together, from the environmental samples in addition to the high molecular homology between the clinical and environmental isolates indicates, at least, regional endemicity of melioidosis in Hainan, China.
数十年来，中国海南已报告有散发性类鼻疽病例；然而，据我们所知，尚无准确的溯源调查证实类鼻疽为地方病。我们确定了4例本地类鼻疽病例，这促使我们采用接触微生物学和分子技术来评估地方病情况。从每位患者住所周围的不同地点采集了环境样本。使用阿什当培养基对样本进行（）筛查，并通过聚合酶链反应和16S核糖体DNA测序进行确认。通过多位点序列分型（MLST）和4位点多位点可变数目串联重复分析（MLVA - 4）对临床和环境分离株进行评估，以确定它们之间的同源性证据。MLST分析表明，一个环境样本和一个临床菌落为序列型46，MLVA - 4分型为（8, 3, 11, 9）型。这些证据表明存在可能的地理和流行病学关联。综合来看，环境样本中的（）以及临床和环境分离株之间的高分子同源性表明，中国海南至少存在类鼻疽的区域地方性流行。
