1 USDA, ARS, Cotton Structure & Quality Research Unit, Southern Regional Research Center (SRRC), New Orleans, LA, USA.
2 USDA, ARS, Cotton Fiber Bioscience Research Unit, Southern Regional Research Center (SRRC), New Orleans, LA, USA.
Appl Spectrosc. 2017 Jul;71(7):1689-1695. doi: 10.1177/0003702816684639. Epub 2017 Jan 20.
The immature fiber ( im) mutant is one type of cotton fiber mutant with unique characteristics of non-fluffy cotton bolls. Compared to its near-isogenic wild type Texas Marker-1 (TM-1), im fiber has a thin secondary cell wall and is less mature. In this work, we applied the previously proposed principal component analysis (PCA) and simple algorithms to analyze the attenuated total reflection Fourier transform infrared (ATR FT-IR) spectra of developmental im and TM-1 fibers. The results from these approaches could not effectively and consistently indicate the inherent difference between TM-1 and im fibers at the same developmental stage. The difference between TM-1 and corresponding im fibers was detected when comparing the normalized intensity variations of the 730 cm bands. The 730 cm band intensities in developmental im fibers are temporally lower than those in developmental TM-1 fibers although they became similar when the TM-1 and im fibers are fully mature. The observation might imply the likelihood of temporal reduction of amorphous regions in developmental im fibers rather than in developmental TM-1 fibers.
不成熟纤维（im）突变体是一种具有独特无绒毛棉铃特征的棉花纤维突变体。与近等基因野生型德克萨斯标记-1（TM-1）相比，im 纤维具有较薄的次生细胞壁，且不太成熟。在这项工作中，我们应用了先前提出的主成分分析（PCA）和简单算法来分析发育中的 im 和 TM-1 纤维的衰减全反射傅里叶变换红外（ATR FT-IR）光谱。这些方法的结果并不能有效地和始终如一地表明在同一发育阶段 TM-1 和 im 纤维之间的内在差异。当比较 730cm 波段的归一化强度变化时，检测到 TM-1 和相应的 im 纤维之间的差异。尽管 TM-1 和 im 纤维完全成熟时，它们变得相似，但发育中的 im 纤维的 730cm 波段强度在时间上低于发育中的 TM-1 纤维。这一观察结果可能意味着在发育中的 im 纤维中，无定形区域的时间减少的可能性比在发育中的 TM-1 纤维中更大。
