Cotton Structure & Quality Research Unit, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), New Orleans, LA 70124, USA.
Cotton Fiber Bioscience Research Unit, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), New Orleans, LA 70124, USA.
Sensors (Basel). 2017 Jun 22;17(7):1469. doi: 10.3390/s17071469.
With cotton fiber growth or maturation, cellulose content in cotton fibers markedly increases. Traditional chemical methods have been developed to determine cellulose content, but it is time-consuming and labor-intensive, mostly owing to the slow hydrolysis process of fiber cellulose components. As one approach, the attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy technique has also been utilized to monitor cotton cellulose formation, by implementing various spectral interpretation strategies of both multivariate principal component analysis (PCA) and 1-, 2- or 3-band/-variable intensity or intensity ratios. The main objective of this study was to compare the correlations between cellulose content determined by chemical analysis and ATR FT-IR spectral indices acquired by the reported procedures, among developmental Texas Marker-1 (TM-1) and immature fiber () mutant cotton fibers. It was observed that the value, , and the integrated intensity of the 895 cm band exhibited strong and linear relationships with cellulose content. The results have demonstrated the suitability and utility of ATR FT-IR spectroscopy, combined with a simple algorithm analysis, in assessing cotton fiber cellulose content, maturity, and crystallinity in a manner which is rapid, routine, and non-destructive.
随着棉纤维的生长或成熟，棉纤维中的纤维素含量显著增加。传统的化学方法已经被开发出来来测定纤维素含量，但这既耗时又费力，主要是由于纤维纤维素成分的水解过程缓慢。作为一种方法，衰减全反射傅里叶变换红外（ATR FT-IR）光谱技术也被用于监测棉纤维素的形成，通过实施多元主成分分析（PCA）和 1-、2-或 3 波段/变量强度或强度比的各种光谱解释策略。本研究的主要目的是比较化学分析确定的纤维素含量与报告程序获得的 ATR FT-IR 光谱指数之间的相关性，这些相关性存在于发育中的德克萨斯标记-1（TM-1）和不成熟纤维（）突变体棉纤维中。观察到值、和 895 cm 波段的积分强度与纤维素含量呈强线性关系。结果表明，ATR FT-IR 光谱结合简单的算法分析，在快速、常规和非破坏性的方式下，适合用于评估棉纤维纤维素含量、成熟度和结晶度。
