使用液相色谱-串联质谱法对人脑脊液中的铁调素-25进行定量分析。

Quantification of hepcidin-25 in human cerebrospinal fluid using LC-MS/MS.

作者信息

Delaby Constance, Bros Pauline, Vialaret Jérôme, Moulinier Amandine, Delatour Vincent, Gabelle Audrey, Lehmann Sylvain, Hirtz Christophe

机构信息

Laboratoire de Biochimie et de Protéomique Clinique - Institut de Médecine Régénérative et Biothérapies (LBPC-IRMB), CHU de Montpellier, 34250 Montpellier, France.

Laboratoire National de Métrologie et d'Essais (LNE), 75015 Paris, France.

出版信息

Bioanalysis. 2017 Feb;9(4):337-347. doi: 10.4155/bio-2016-0240. Epub 2017 Jan 20.

DOI:10.4155/bio-2016-0240

PMID:28106476

Abstract

AIM

Hepcidin, the main iron metabolism regulator, can be detected in various biological fluids. Here, we describe a quantitative method of LC-MS/MS to quantify the 25 amino acid isoform of hepcidin (hepcidin-25) in human cerebrospinal fluid (CSF). Results & methodology: Samples were prepared through protein precipitation followed by solid phase extraction (SPE) and injected into a triple-quadrupole mass spectrometer. Validation of our method included determination of LOQ (0.1 ng/ml), repeatability, intermediate precision, recovery and linearity (up to 25 ng/ml). Hepcidin-25 was subsequently quantified in 36 human CSF samples and its concentration ranged from 0.21 to 3.54 ng/ml.

CONCLUSION

This is the first time that hepcidin-25 can be reliably quantified in human CSF. This may open interesting perspectives for the management of iron-related neurological disorders.

摘要

目的

铁调素是主要的铁代谢调节因子，可在多种生物体液中检测到。在此，我们描述一种液相色谱-串联质谱法定量检测人脑脊液（CSF）中铁调素25个氨基酸异构体（铁调素-25）的方法。结果与方法：样品经蛋白沉淀后进行固相萃取（SPE），然后注入三重四极杆质谱仪。该方法的验证包括定量限（0.1 ng/ml）的测定、重复性、中间精密度、回收率和线性（高达25 ng/ml）。随后对36份人CSF样品中的铁调素-25进行定量，其浓度范围为0.21至3.54 ng/ml。