Bcl-2 编码区单核苷酸多态性与结直肠癌发生及预后的关系：一项病例对照研究。

Association of single nucleotide polymorphisms in the coding region of Bcl-2 with the occurrence and prognosis of colorectal cancer: A case-control study.

机构信息

Department of Laboratory, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China.

Department of Pediatrics, Yantai Yuhuangding Hospital, Yantai 264000, Shandong, China.

出版信息

Cancer Biomark. 2017;18(4):433-439. doi: 10.3233/CBM-160378.

DOI:10.3233/CBM-160378

PMID:28106544

Abstract

OBJECTIVE

To investigate the relationship of single nucleotide polymorphisms in the coding region of Bcl-2 with the occurrence and prognosis of colorectal cancer (CRC).

METHODS

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used detect Bcl-2 gene polymorphisms (rs1800477A/G and rs1801018A/G) in 185 patients with CRC (case group) and 177 healthy subjects (control group). The relationships of Bcl-2 gene polymorphisms with clinicopathological features and prognosis of CRC patients were analyzed.

RESULTS

The frequency of GG genotype and G allele of rs1800477A/G in the case group were significantly higher than those in the control group (both P < 0.05). The GG genotype of rs1800477A/G was associated with lymph node metastasis and Dukes' staging of CRC (both P < 0.05). Haplotype analysis demonstrated that the case group had decreased frequency of GA haplotype, but increased frequency of GG haplotype in comparisons to the control group (GA: P = 0.014; GG: P = 0.003). Kaplan-Meier survival analysis showed that the risk of death (within 5 years) in patients carrying GG genotype (rs1800477A/G) was 2.159 as much as that in patients carrying AA + GA genotype. Multivariate analyses showed that Dukes' staging and GG genotype of rs1800477A/G are risk factors for poor prognosis in CRC (Dukes' staging: P = 0.001; GG genotype: P = 0.034).

CONCLUSION

Bcl-2 rs1800477A/G polymorphism may be related to the occurrence of CRC, and GG genotype could be a risk factor of poor prognosis in CRC.

摘要

目的

探讨 Bcl-2 编码区单核苷酸多态性与结直肠癌（CRC）发生发展及预后的关系。

方法

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测 185 例 CRC 患者（病例组）和 177 例健康对照者（对照组）的 Bcl-2 基因多态性（rs1800477A/G 和 rs1801018A/G），分析 Bcl-2 基因多态性与 CRC 患者临床病理特征及预后的关系。

结果

病例组 rs1800477A/G 中 GG 基因型和 G 等位基因频率显著高于对照组（均 P<0.05）。rs1800477A/G 的 GG 基因型与 CRC 的淋巴结转移和 Dukes 分期有关（均 P<0.05）。单体型分析显示，与对照组相比，病例组 GA 单体型频率降低，而 GG 单体型频率增加（GA：P=0.014；GG：P=0.003）。Kaplan-Meier 生存分析显示，携带 GG 基因型（rs1800477A/G）的患者死亡风险（5 年内）是携带 AA+GA 基因型患者的 2.159 倍。多因素分析显示，Dukes 分期和 rs1800477A/G 的 GG 基因型是 CRC 预后不良的危险因素（Dukes 分期：P=0.001；GG 基因型：P=0.034）。