Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China.
Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, PR China.
Talanta. 2017 Mar 1;164:27-33. doi: 10.1016/j.talanta.2016.10.100. Epub 2016 Nov 5.
Single or few-layer nanosheets of MoS (MoS nanosheets) and a composite composed of MoS nanosheets, Au nanoparticles (AuNPs) and hemin (HE) (denoted as MoS-Au-HE) were prepared. The composites possessed high synergetic catalysis activity towards the electroreduction of hydrogen peroxide. Furthermore, glucose oxidase (GOD) and AuNPs were used as marker of the complementary DNA (cDNA) strand of kanamycin aptamer to prepare a conjugate (reffered as cDNA-Au-GOD) that was designed as the signal probe. Both cDNA-Au-GOD and MoS-Au-HE were applied to fabricate aptasensor for kanamycin. MoS-Au-HE acted as solid platform for kanamycin aptamer and signal transmitters. AuNPs were employed as the supporter of cDNA and GOD which catalyze dissolved oxygen to produce hydrogen peroxide in the presence of glucose. Then cathodic peak current of HO was recorded by differential pulse voltammetry (DPV). The electrochemical reduction of HO was catalyzed by MoS-Au-HE that was modified onto the surface of a glassy carbon electrode (GCE). The cathodic peak current of HO was highly linearly decreased with an increase of kanamycin concentrations from 1.0ng/L to 1.0×10ng/L, with a detection limit of 0.8ng/L. This aptasensor can be used to detect kanamycin in milk with high specificity, sensitivity and selectivity.
制备了单层或少数层的 MoS（MoS 纳米片）和由 MoS 纳米片、Au 纳米粒子（AuNPs）和血红素（HE）组成的复合材料（表示为 MoS-Au-HE）。该复合材料对过氧化氢的电还原具有高协同催化活性。此外，葡萄糖氧化酶（GOD）和 AuNPs 被用作庆大霉素适体互补 DNA（cDNA）链的标记物，以制备被设计为信号探针的缀合物（称为 cDNA-Au-GOD）。cDNA-Au-GOD 和 MoS-Au-HE 均用于制备庆大霉素适体传感器。MoS-Au-HE 充当庆大霉素适体和信号转导器的固体平台。AuNPs 被用作 cDNA 和 GOD 的载体，在存在葡萄糖的情况下，它们将溶解氧催化为过氧化氢。然后通过差分脉冲伏安法（DPV）记录 HO 的阴极峰电流。HO 的电化学还原由修饰在玻碳电极（GCE）表面的 MoS-Au-HE 催化。随着庆大霉素浓度从 1.0ng/L 增加到 1.0×10ng/L，HO 的阴极峰电流呈高度线性下降，检测限为 0.8ng/L。该适体传感器可用于检测牛奶中的庆大霉素，具有高特异性、灵敏度和选择性。
