Ohuchi N, Takahashi K, Matoba N, Sato T, Taira Y, Sakai N, Masuda M, Mori S
Second Department of Surgery, Tohoku University School of Medicine, Sendai.
Jpn J Clin Oncol. 1989 Sep;19(3):242-8.
Tumor-associated glycoprotein (TAG-72) has been shown to be expressed in a wide variety of epithelial malignant tissues. We have investigated serum levels of TAG-72 antigen in patients with gastrointestinal cancer with a solid phase radioimmunometric assay (RIA), CA72-4, utilizing murine monoclonal antibodies CC49 and B72.3 which recognize the TAG-72 antigen. Elevated levels of serum TAG-72 antigen were found in 48% of 56 gastric carcinoma patients and 67% of 45 colorectal carcinoma patients. The serum concentrations of TAG-72 were compared to those of CA19-9 and CEA. The positive rates of CA19-9 in gastric carcinoma and colorectal carcinoma patients were 29% and 54%, and those of CEA were 52% and 60%, respectively. Elevated serum levels of TAG-72, CA19-9 and CEA were observed in 7%, 14% and 24%, respectively, of patients with benign disease, thus indicating a preferential expression of TAG-72, compared to CA19-9 and CEA, in gastrointestinal carcinoma patients versus in patients with benign disorder. A cocktail of CA72-4, CA19-9 and CEA RIAs increased positive rates to 68% in sera of gastric cancer patients and 84% in sera of colorectal cancer patients. Combination assays using CA72-4, CEA and CA19-9 RIAs for patients with benign gastrointestinal disorder, however, also increased the positive rate to 31%. These results indicate that CA72-4, CA19-9 and CEA RIAs may be complementary in detecting circulating tumor-associated antigens. It must be emphasized, however, that interpretation of the data provided by the combination serum assays requires careful consideration.
肿瘤相关糖蛋白（TAG - 72）已被证明在多种上皮性恶性组织中表达。我们利用固相放射免疫测定法（RIA）CA72 - 4，使用识别TAG - 72抗原的鼠单克隆抗体CC49和B72.3，对胃肠道癌患者血清中TAG - 72抗原水平进行了研究。在56例胃癌患者中有48%以及45例结直肠癌患者中有67%发现血清TAG - 72抗原水平升高。将TAG - 72的血清浓度与CA19 - 9和癌胚抗原（CEA）的浓度进行了比较。胃癌和结直肠癌患者中CA19 - 9的阳性率分别为29%和54%，CEA的阳性率分别为52%和60%。良性疾病患者中分别有7%、14%和24%观察到血清TAG - 72、CA19 - 9和CEA水平升高，这表明与CA19 - 9和CEA相比，TAG - 72在胃肠道癌患者中相对于良性疾病患者有优先表达。CA72 - 4、CA19 - 9和CEA RIA联合检测使胃癌患者血清阳性率提高到68%，结直肠癌患者血清阳性率提高到84%。然而，对于良性胃肠道疾病患者，使用CA72 - 4、CEA和CA19 - 9 RIA的联合检测也使阳性率提高到31%。这些结果表明，CA72 - 4、CA19 - 9和CEA RIA在检测循环肿瘤相关抗原方面可能具有互补性。然而，必须强调的是，对联合血清检测所提供数据的解读需要仔细考虑。
