Zheng Xiaoting, Duan Yafei, Dong Hongbiao, Zhang Jiasong
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, PR China; College of Fisheries & Life Science, Shanghai Ocean University, Shanghai, 201306, PR China.
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, PR China.
Fish Shellfish Immunol. 2017 Mar;62:195-201. doi: 10.1016/j.fsi.2017.01.015. Epub 2017 Jan 17.
A 45-day feeding trial followed by an acute stress test of low salinity was done to evaluate effects of Lactobacillus plantarum on growth performance and anti-stress capability of white shrimp (Litopenaeus vannamei). Shrimp were randomly allocated in 15 tanks (100 shrimp per tank) and divided into 5 treatments with 3 replicates. Triplicate tanks were fed with a control diet or diets containing different treatments of L. plantarum (fermentation supernatant (FS), live bacteria (LB), dead bacteria (DB) and cell-free extract (CE) of L. plantarum) as treatment groups. Growth performance including weight gain (WGR), feed conversion ratio (FCR) and specific growth rate (SGR) were determined after feeding 45 days. Anti-stress capacity was evaluated by determining the gene expression of ProPO, SOD and Lys in gut of shrimp at the end of feeding trial and again at 96 h post-stress test. Results indicated that supplementation of L. plantarum into diet had significantly improved growth performance of L. vannamei. On the other hand, L. plantarum supplementation had no significant effects on the gene expression of SOD and Lys in gut of shrimp cultured under normal condition for 45 days. Supplementation of L. plantarum had increased survivability of L. vannamei having higher survival rates compared to the control group. However, statistical analysis showed no significant difference between the control group and treatments. Compared with the control group, supplementation of L. plantarum significantly improved the resistance of L. vannamei against the stress of acute low salinity, as indicated by higher survival rate as well as higher transcript levels of ProPo, SOD and Lys gene. Our findings suggested that L. plantarum, especially cell-free extract of L. plantarum has improved the anti-stress capacity of L. vannamei and could serve as a potential feed additive that helps shrimp to overcome environmental stresses.
进行了一项为期45天的投喂试验，随后进行低盐度急性应激试验，以评估植物乳杆菌对凡纳滨对虾生长性能和抗应激能力的影响。将虾随机分配到15个水箱中（每个水箱100只虾），分为5个处理组，每组3个重复。将三个重复水箱分别投喂对照饲料或含有不同处理的植物乳杆菌（植物乳杆菌发酵上清液（FS）、活细菌（LB）、死细菌（DB）和无细胞提取物（CE））的饲料作为处理组。投喂45天后测定生长性能，包括增重率（WGR）、饲料转化率（FCR）和特定生长率（SGR）。在投喂试验结束时以及应激试验后96小时，通过测定虾肠道中ProPO、SOD和Lys的基因表达来评估抗应激能力。结果表明，在饲料中添加植物乳杆菌显著提高了凡纳滨对虾的生长性能。另一方面，在正常条件下养殖45天的虾肠道中，添加植物乳杆菌对SOD和Lys的基因表达没有显著影响。添加植物乳杆菌提高了凡纳滨对虾的存活率，与对照组相比存活率更高。然而，统计分析表明对照组和处理组之间没有显著差异。与对照组相比，添加植物乳杆菌显著提高了凡纳滨对虾对急性低盐度应激的抵抗力，表现为更高的存活率以及ProPo、SOD和Lys基因更高的转录水平。我们的研究结果表明，植物乳杆菌，尤其是植物乳杆菌无细胞提取物提高了凡纳滨对虾的抗应激能力，可作为一种潜在的饲料添加剂，帮助对虾克服环境应激。
