Guangdong Provincial Key Laboratory of Improved Variety Reproduction in Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Improved Variety Reproduction in Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
Fish Shellfish Immunol. 2018 Sep;80:452-457. doi: 10.1016/j.fsi.2018.06.039. Epub 2018 Jun 20.
A 25-days experiment was conducted to evaluate the effect of dietary Haematococcus pluvialis on growth, survival, immune response and stress tolerance ability of post-larval Litopenaeus vannamei. Post-larval white shrimp (mean initial weight 2.1 mg) were fed five isoenergic and isonitrogenous diets containing grade levels of Haematococcus pluvialis (0, 1.7, 3.3, 6.7 and 13.3 g kg diet, respectively). Results indicated that 3.3 g Haematococcus pluvialis kg diet increased the survival rate of post-larval white shrimp. Specific growth rate (SGR) and weight gain (WG) showed no difference among each groups. After the acute salinity stress (salinity decreased rapidly from 28‰ to 5‰), survival of shrimp fed 6.7 g Haematococcus pluvialis kg diet significant higher than the control (P < 0.05), and the total antioxidant capacity (T-AOC) was increased with the increasing dietary Haematococcus pluvialis levels. The malonaldehyde (MDA) contents in whole body decreased with the increasing dietary Haematococcus pluvialis levels before and after the salinity stress. Before the salinity stress, relative mRNA levels of Caspase 3, Rho and Janus kinase (JAK) decreased in shrimp fed diets contain Haematococcus pluvialis. After the salinity stress, relative mRNA levels of anti-oxidative related genes and immune related genes decreased with the dietary Haematococcus pluvialis level increased to 3.3 g kg. Based on the effect of Haematococcus pluvialis on survival, salinity stress tolerance ability and the immune response of post-larval L. vannamei, the optimal level of Haematococcus pluvialis was 3.3-6.7 g kg diet (100-200 mg astaxanthin kg diet).
进行了一项为期 25 天的实验，以评估饲料雨生红球藻对凡纳滨对虾幼虾生长、存活、免疫反应和应激耐受能力的影响。幼虾（平均初始体重 2.1mg）喂食五组等能量和等氮的饲料，其中雨生红球藻的含量分别为 0、1.7、3.3、6.7 和 13.3g/kg 饲料。结果表明，3.3g/kg 饲料的雨生红球藻可提高幼虾的存活率。特定生长率（SGR）和体重增加（WG）在各组之间没有差异。在急性盐度胁迫（盐度迅速从 28‰降至 5‰）后，摄食 6.7g/kg 饲料的虾的存活率显著高于对照组（P<0.05），且总抗氧化能力（T-AOC）随饲料雨生红球藻水平的增加而增加。盐度胁迫前后，丙二醛（MDA）含量随饲料雨生红球藻水平的增加而降低。盐度胁迫前，摄食含雨生红球藻饲料的虾 Caspase 3、Rho 和 Janus 激酶（JAK）的相对 mRNA 水平降低。盐度胁迫后，随着饲料雨生红球藻水平增加到 3.3g/kg，抗氧化相关基因和免疫相关基因的相对 mRNA 水平降低。基于雨生红球藻对凡纳滨对虾幼虾存活率、耐盐能力和免疫反应的影响，雨生红球藻的最佳水平为 3.3-6.7g/kg 饲料（100-200mg 虾青素/kg 饲料）。
