Maeda H, Tanaka N, Ohshima T, Nagano T
Nihon Hoigaku Zasshi. 1989 Jun;43(3):238-42.
The ultrastructural changes in human erythrocytes caused by heat and the localization of ABO blood group activity have been studied electron microscopically. Osmotically hemolyzed cells were found to show varying degrees of cell matrix loss and a swelling of the membranes. By means of immunoferritin staining, ferritin particles, which indicate the localization of ABO group activity, were found to bind in clusters on small process-like structures on the membranes. Erythrocytes heated to 60 degrees C showed a hemolytic change; the cell matrix was released and small globular substances (ca. 50-100 nm in diameter) aggregated on the cytoplasmic side of the partially swollen cell membranes, a phenomenon that was not noted in erythrocytes that undergo a hypo-osmotic change. Erythrocytes heated to 100 degrees C underwent fragmentation, though the cell matrix was almost preserved and aggregated substances of varying size were noted inside the membranes. ABO group activity was demonstrated almost evenly on the sub-membrane aggregated substances and on the surface of the thermo-changed erythrocytes.
通过电子显微镜研究了热对人红细胞超微结构的影响以及ABO血型活性的定位。发现经渗透溶血的细胞呈现出不同程度的细胞基质丢失和细胞膜肿胀。通过免疫铁蛋白染色，发现指示ABO血型活性定位的铁蛋白颗粒聚集结合在细胞膜上小的突起样结构上。加热到60℃的红细胞出现溶血变化；细胞基质被释放，小的球状物质（直径约50 - 100nm）聚集在部分肿胀细胞膜的细胞质侧，这种现象在经历低渗变化的红细胞中未观察到。加热到100℃的红细胞发生破碎，尽管细胞基质几乎保留，且在膜内可见大小不一的聚集物质。ABO血型活性在膜下聚集物质和热改变红细胞表面几乎均匀显示。
