Maeda H, Tanaka N, Fukui H, Nakaya T, Nagano T
Nihon Hoigaku Zasshi. 1989 Aug;43(4):287-93.
Changes in the macroscopic appearance and in the activity of various erythrocyte antigens were observed by heating human erythrocytes of known blood groups at 60-260 degrees C for 15, 30, and 60 min in a suspension that was sealed in an airtight test tube. While the erythrocytes underwent severe hemolysis after heating at 60 degrees C, this phenomenon was not macroscopically observed on heating at 80 and 100 degrees C and the cellular sludge in the tested became brown in color. With further temperature increases, the supernatant became pale yellow at 120-140 degrees C, brown at 160 degrees C and dark brown at 180 degrees C and above, whereas the sediment hardened and became darker in tone. Both A and B activity in the cellular sludge remained active until a 30-min heating at 240 degrees C, as did the O(H) activity until a 15-min heating at 220 degrees C. Both Lewis type activity was detected until a 30-min heating at 200 degrees C and a 15-min heating at 220 degrees C. The P1 activity remained specific after a 30-min heating at 180 degrees C and false-positive reactions were observed after 60 min at 180 degrees C. Among the Rh antigen activity, D activity remained until a 60-min heating at 140 degrees C, C and E until a 30-min heating at 140 degrees C, c until a 15-min heating at 140 degrees C and e until a 30-min heating at 100 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)
通过将已知血型的人红细胞在密封于气密试管的悬浮液中于60 - 260℃加热15、30和60分钟，观察其宏观外观和各种红细胞抗原活性的变化。在60℃加热后红细胞发生严重溶血，但在80℃和100℃加热时未观察到这种宏观现象，测试中的细胞沉淀变为褐色。随着温度进一步升高，在120 - 140℃上清液变为浅黄色，160℃时变为褐色，180℃及以上变为深褐色，而沉淀变硬且颜色变深。细胞沉淀中的A和B活性在240℃加热30分钟前均保持活跃，O(H)活性在220℃加热15分钟前保持活跃。两种Lewis型活性在200℃加热30分钟和220℃加热15分钟前均可检测到。P1活性在180℃加热30分钟后仍保持特异性，在180℃加热60分钟后出现假阳性反应。在Rh抗原活性中，D活性在140℃加热60分钟前保持，C和E在140℃加热30分钟前保持，c在140℃加热15分钟前保持，e在100℃加热30分钟前保持。（摘要截于250字）
