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[利用铁蛋白和金标记的苹果螺保护素对人红细胞血型抗原A进行电子显微镜检测]

[Electron microscopic detection of blood group antigen A on human erythrocytes by means of ferritin- and gold-labelled protectin of helix pomatia].

作者信息

Wagner M, Wagner B

出版信息

Z Immunitatsforsch Exp Klin Immunol. 1976 Apr;151(2):117-25.

PMID:134531
Abstract

By means of ferritin- and gold-labelled protectin from the albumen gland of the edible snail Helix pomatia the blood group antigen A was located on human erythrocytes of groups A1, A2 and A1B. With erythrocytes of groups O and B the reaction is negative. The antigen is focally distributed on the outer surface of the cell membrane. Cells from groups A1 and A1B have an antigen A concentration about 4 times greater than A2 cells. The numbers of particles after tagging with ferritin or gold are comparable. The gold particles show an extremely high contrast and are therefore very suitable for the immunoelectron microscopic localization of antigens.

摘要

借助来自可食用蜗牛玛瑙螺蛋白腺的铁蛋白和金标记的保护素，在A1、A2和A1B血型的人红细胞上定位了血型抗原A。O型和B型红细胞的反应为阴性。该抗原在细胞膜外表面呈局灶性分布。A1和A1B型细胞的抗原A浓度约为A2型细胞的4倍。用铁蛋白或金标记后的颗粒数量相当。金颗粒显示出极高的对比度，因此非常适合抗原的免疫电子显微镜定位。

相似文献

1
[Electron microscopic detection of blood group antigen A on human erythrocytes by means of ferritin- and gold-labelled protectin of helix pomatia].[利用铁蛋白和金标记的苹果螺保护素对人红细胞血型抗原A进行电子显微镜检测]
Z Immunitatsforsch Exp Klin Immunol. 1976 Apr;151(2):117-25.
2
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Z Immunitatsforsch Immunobiol. 1977 Dec;153(5):450-6.
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[Use of an agglutinin from the albumin gland of the edible snail for immunoelectron microscopic localization of cell membrane antigens with terminal n-acetyl-d-galactosamine].[利用可食用蜗牛白蛋白腺中的凝集素对具有末端N-乙酰-D-半乳糖胺的细胞膜抗原进行免疫电子显微镜定位]
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Gold particles coated with anti-A versus ferritin or 125iodine-labeled anti-A in reactions with cell surface antigen.在与细胞表面抗原的反应中，包被有抗A的金颗粒与铁蛋白或125碘标记的抗A相对比。
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