College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Bioinformatics Center, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Microbiologyopen. 2017 Jun;6(3). doi: 10.1002/mbo3.436. Epub 2017 Jan 21.
We used the goat as a model to study the effects of rumen microbial composition and epithelial TLR signaling on maintaining rumen stability during exogenous butyrate interference. Six cannulated goats received a rapid intraruminal infusion of 0.1 mol/L potassium phosphate buffer with (BT, n = 3) or without (CO, n = 3) 0.3 g/kg·BW·day sodium butyrate for 28 days. The ruminal pH and the concentration of total SCFA were not affected by the interference. 16S rRNA gene amplicon sequencing revealed a change in microbial composition after the butyrate infusion. LEfSe analysis showed a shift of the biomarker species from butyrate-producing bacteria to acetate-and propionate-producing bacteria. Quantitative PCR-based comparisons showed that significant increases in TLR2, TLR5, and MyD88 expression were accompanied by a significant decrease in IL-1β and IFN-γ expression in the ruminal epithelium. Constrained correlation analysis showed that the relative abundance of Roseburia was positively correlated with the expression of TLR5. Taken together, our study shows that microbial composition plays an important role in maintaining the stability of the microbial ecosystem in rumen, and indicates that the microbe-TLR-cytokine axis was involved in maintaining the stability of the gastrointestinal ecosystem.
我们使用山羊作为模型，研究了瘤胃微生物组成和上皮 TLR 信号在丁酸干扰过程中维持瘤胃稳定性方面的作用。六只有套管的山羊在 28 天内接受快速瘤胃内输注 0.1 mol/L 磷酸钾缓冲液，其中（BT，n=3）或不添加（CO，n=3）0.3 g/kg·BW·天的丁酸钠。干扰后，瘤胃 pH 值和总 SCFA 浓度不受影响。16S rRNA 基因扩增子测序显示丁酸输注后微生物组成发生变化。LEfSe 分析显示，生物标志物从丁酸产生菌向乙酸和丙酸产生菌转移。基于定量 PCR 的比较显示，TLR2、TLR5 和 MyD88 表达显著增加，同时瘤胃上皮中 IL-1β 和 IFN-γ 表达显著降低。约束相关分析表明，Roseburia 的相对丰度与 TLR5 的表达呈正相关。总之，我们的研究表明，微生物组成在维持瘤胃微生物生态系统的稳定性方面起着重要作用，并表明微生物-TLR-细胞因子轴参与维持胃肠道生态系统的稳定性。
