College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Bioinformatics Center, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Microbiologyopen. 2018 Feb;7(1). doi: 10.1002/mbo3.537. Epub 2017 Oct 5.
We used 16S rRNA gene sequencing to examine the posteffects of antibiotic treatment on the structure and metabolism of rumen microbiota. Twelve goats were randomly assigned into two groups, with one group receiving intramuscular streptomycin injection at 40 mg/kg bodyweight daily for 10 days. At 4 weeks after treatment with antibiotic, three goats were randomly selected from each group and switched to a 35% concentrate diet. The remaining six goats continued with the 10% concentrate diet. At 4 weeks after dietary shift, ruminal fluid and epithelium were collected to analyze the microbiota composition and short-chain fatty acid (SCFA) concentrations of the rumen. We found that antibiotic administration led to increases in the diversity and richness of recovered mucosal microbiota and to decreases in those of recovered fluid microbiota. When dietary modulation was performed after antibiotic intake, both communities showed little difference in structure from premodulated states. Additionally, antibiotic pretreatment reduced the basal lines of individual SCFAs but did not affect the increased percentages of SCFAs. Overall, our results indicate that antibiotic administration affects the structure of both rumen fluid and mucosal microbiota and reduces the functional redundancy of rumen microbiota.
我们使用 16S rRNA 基因测序来研究抗生素治疗对瘤胃微生物区系结构和代谢的后效。将 12 只山羊随机分为两组，一组每天肌肉注射 40mg/kg 体重的链霉素，连续 10 天。抗生素治疗 4 周后，每组随机选择 3 只羊转为 35%浓缩饲料。其余 6 只羊继续使用 10%浓缩饲料。饲料转换 4 周后，采集瘤胃液和黏膜上皮，分析瘤胃微生物组成和短链脂肪酸（SCFA）浓度。我们发现，抗生素给药导致恢复黏膜微生物区系的多样性和丰富度增加，而恢复液微生物区系的多样性和丰富度降低。当抗生素摄入后进行饮食调节时，两个群落的结构与预先调节的状态相比几乎没有差异。此外，抗生素预处理降低了个体 SCFA 的基线水平，但不影响 SCFA 增加的百分比。总的来说，我们的结果表明，抗生素给药会影响瘤胃液和黏膜微生物区系的结构，并降低瘤胃微生物区系的功能冗余。
