Takase H, Imanishi K, Miura O, Yumioka E
Kampo Research Laboratories, Kanebo, Ltd., Osaka, Japan.
Jpn J Pharmacol. 1989 Sep;51(1):17-23. doi: 10.1254/jjp.51.17.
We investigated the involvement of sulfhydryl-compounds in the cytoprotective effect of Oren-gedoku-to (OGT) against ethanol-induced gastric lesions and potential difference (PD) reduction in comparison with those of sucralfate and glutathione in rats. Pretreatment with indomethacin (IND) had little influence on the cytoprotective effects of OGT and glutathione, but attenuated the effect of sucralfate. Pretreatment with N-ethylmaleimide (NEM) blocked the cytoprotective effects of these three drugs. Pretreatment with iodoacetamide diminished the cytoprotection of OGT and glutathione, but had little effect on that of sucralfate. The cytoprotective activities of OGT, glutathione and sucralfate were little affected by pretreatment with 6,6'-dithiodinicotinic acid. The inhibitory effects of OGT and glutathione against the PD reduction were completely blocked by pretreatment with NEM, while they were not influenced by pretreatment with IND. On the other hand, the inhibitory effect of sucralfate disappeared both by IND and NEM. These results suggest that the cytoprotective effect of OGT may be mediated by endogenous sulfhydryl compounds, but not by endogenous prostaglandins in the gastric mucosa.
我们研究了与硫糖铝和谷胱甘肽相比，黄连解毒汤（OGT）对乙醇诱导的大鼠胃损伤及电位差（PD）降低的细胞保护作用中巯基化合物的参与情况。吲哚美辛（IND）预处理对OGT和谷胱甘肽的细胞保护作用影响不大，但减弱了硫糖铝的作用。N - 乙基马来酰亚胺（NEM）预处理阻断了这三种药物的细胞保护作用。碘乙酰胺预处理减弱了OGT和谷胱甘肽的细胞保护作用，但对硫糖铝的作用影响不大。6,6'-二硫代二烟酸预处理对OGT、谷胱甘肽和硫糖铝的细胞保护活性影响较小。NEM预处理完全阻断了OGT和谷胱甘肽对PD降低的抑制作用，而IND预处理对其无影响。另一方面，硫糖铝的抑制作用在IND和NEM预处理后均消失。这些结果表明，OGT的细胞保护作用可能由胃黏膜中的内源性巯基化合物介导，而非内源性前列腺素。
