Nalloor Thomas John Philip, Kumar Nitesh, Narayanan Kasinathan, Palanimuthu Vasanth Raj
Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, Karnataka.
Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, Karnataka.
J Basic Clin Physiol Pharmacol. 2017 May 1;28(3):257-265. doi: 10.1515/jbcpp-2016-0058.
Butter is one of the widely used fats present in the diet. However, there is no satisfactory study available that evaluates the effect of a high-fat diet containing butter as the principal fat on the development of non-alcoholic fatty liver disease (NAFLD).
In the present study, butter was used for the development of steatosis in Chang liver cells in an in vitro study and Swiss albino mice in an in vivo study. In vitro steatosis was established, and butter was compared with oleic acid in Chang liver cells using an oil red O (ORO)-based colorimetric assay. In the in vivo study, a butter-rich special diet was fed for 15 weeks to mice, who showed no significant change in body weight. The expression pattern of phosphatase and tensin homolog (PTEN) and miR-21 was compared by reverse transcriptase-PCR.
Special diet-fed animals showed downregulated PTEN compared to normal diet-fed animals, while levels of miR-21 remained the same. Elevations in biochemical parameters, viz., triglycerides and liver function tests showed symptoms of onset of NAFLD. Histophathological study of livers of test animals confirmed mild-to-moderate degree of NAFLD.
黄油是饮食中广泛使用的脂肪之一。然而，尚无令人满意的研究评估以黄油作为主要脂肪的高脂肪饮食对非酒精性脂肪性肝病（NAFLD）发展的影响。
在本研究中，在体外研究中使用黄油在 Chang 肝细胞中诱导脂肪变性，在体内研究中使用瑞士白化小鼠。建立体外脂肪变性模型，并使用基于油红 O（ORO）的比色法在 Chang 肝细胞中将黄油与油酸进行比较。在体内研究中，给小鼠喂食富含黄油的特殊饮食 15 周，小鼠体重无明显变化。通过逆转录聚合酶链反应比较磷酸酶和张力蛋白同源物（PTEN）和 miR-21 的表达模式。
与正常饮食喂养的动物相比，特殊饮食喂养的动物 PTEN 表达下调，而 miR-21 水平保持不变。生化参数（即甘油三酯和肝功能测试）升高显示出 NAFLD 发病的症状。对试验动物肝脏的组织病理学研究证实为轻度至中度 NAFLD。
