Department of Nutritional Sciences, R.F. Molecular Nutritional Science, University of Vienna, Vienna, Austria.
Institute of Nutrition, SD Model Systems of Molecular Nutrition, Friedrich-Schiller University of Jena, Jena, Germany.
Metabolism. 2020 Aug;109:154283. doi: 10.1016/j.metabol.2020.154283. Epub 2020 Jun 1.
Absolute dietary fat intake but even more so fatty acid pattern is discussed to be critical in the development of non-alcoholic fatty liver disease (NAFLD). Here, we determined if switching a butterfat enriched diet to a rapeseed oil (RO) enriched diet affects progression of an existing NAFLD and glucose intolerance in mice.
For eight weeks, female C57Bl/6J mice were either fed a liquid control (C) or a butterfat-, fructose- and cholesterol-rich diet (BFC, 25E% butterfat) to induce early signs of steatohepatitis and glucose intolerance in mice. For additional five weeks mice received either BFC or C or a fat-, fructose- and cholesterol-rich and control diet, in which butterfat was replaced with RO (ROFC and CRO). Markers of glucose metabolism, liver damage and intestinal barrier were assessed.
Exchanging butterfat with RO attenuated the progression of BFC diet-induced NAFLD and glucose intolerance. Beneficial effects of RO were associated with lower portal endotoxin levels and an attenuation of the induction of the toll-like receptor-4-dependent signaling cascades in liver. Peroxisome proliferator-activated receptor γ activity was induced in small intestine of ROFC-fed mice.
Taken together, exchanging butterfat with RO attenuated the progression of diet-induced steatohepatitis and glucose intolerance in mice.
绝对的脂肪摄入量，甚至脂肪酸模式，都被认为在非酒精性脂肪肝（NAFLD）的发展中至关重要。在这里，我们确定了用富含菜籽油（RO）的饮食代替富含乳脂的饮食是否会影响现有的 NAFLD 和小鼠的葡萄糖不耐受的进展。
八周内，雌性 C57Bl/6J 小鼠要么喂食液体对照（C），要么喂食富含乳脂、果糖和胆固醇的饮食（BFC，25E%乳脂），以诱导小鼠出现肝脂肪变性和葡萄糖不耐受的早期迹象。另外五周，小鼠接受 BFC 或 C 或高脂肪、果糖和胆固醇且对照饮食，其中乳脂用 RO 代替（ROFC 和 CRO）。评估葡萄糖代谢、肝损伤和肠道屏障的标志物。
用 RO 代替乳脂可减轻 BFC 饮食诱导的 NAFLD 和葡萄糖不耐受的进展。RO 的有益作用与门脉内毒素水平降低以及肝脏中 Toll 样受体 4 依赖性信号级联反应的诱导减弱有关。ROFC 喂养的小鼠小肠中过氧化物酶体增殖物激活受体γ活性增加。
总之，用 RO 代替乳脂可减轻饮食诱导的小鼠肝脂肪变性和葡萄糖不耐受的进展。
