硫代乙酰胺（TAM）对NADP连接酶、氨基转移酶和谷氨酸脱氢酶的肝毒性作用。
Hepatotoxic effect of thioacetamide (TAM) on NADP-linked enzymes, aminotransferases and glutamate dehydrogenase.
作者信息
Cerdán S, Cascales M, Chacón P, Cascales F, Santos-Ruiz A
出版信息
Arch Toxicol Suppl. 1978(1):221-4. doi: 10.1007/978-3-642-66896-8_39.
NADP-linked dehydrogenases, glucose-6-P dehydrogenase (G 6PDH) 6-P gluconate dehydrogenase (6 PGDH), isocitrate dehydrogenase (ICDH), malate dehydrogenase decarboxylating (ME) and aminotransferases GOT and GPT were analyzed in the soluble fraction of blood free homogenates. Glutmate dehydrogenase (GDH) was assayed in the mitochondrial fraction. TAM was i.p. administered to male albino rats (50 mg/kg/day) for 28 days. enzyme activities were determined as described by Bermeyer 1965 (Methods Enzymatic Analysis. Verlag Chemie. Acad. Press).
分析了无细胞匀浆可溶性部分中的烟酰胺腺嘌呤二核苷酸磷酸（NADP）连接的脱氢酶、葡萄糖-6-磷酸脱氢酶（G 6PDH）、6-磷酸葡萄糖酸脱氢酶（6 PGDH）、异柠檬酸脱氢酶（ICDH）、脱羧苹果酸脱氢酶（ME）以及转氨酶谷草转氨酶（GOT）和谷丙转氨酶（GPT）。谷氨酸脱氢酶（GDH）在线粒体部分进行测定。将三乙酰吗啉（TAM）腹腔注射给雄性白化大鼠（50毫克/千克/天），持续28天。酶活性按照1965年贝迈尔所述方法测定（《酶学分析方法》。化学出版社。学术出版社）。
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