El-Deeb Wael, Almujalli Abdul Aziz, Eljalii Isam, Elmoslemany Ahmed, Fayez Mahmoud
Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Ahsa, Saudi Arabia; Department of Veterinary Medicine, Infectious Disease, and Fish Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Department of Clinical Studies, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Ahsa, Saudi Arabia.
Acta Trop. 2017 Apr;168:74-79. doi: 10.1016/j.actatropica.2017.01.017. Epub 2017 Jan 19.
Classical contagious caprine pleuropneumonia is one of the most fatal contagious disease of goats listed by World Organization for Animal Health that leads to major economic losses. It is caused by infection with Mycoplasma capricolum subspecies capripneumoniae. In order to isolate the causative agents of CCPP for the first time in the Kingdom of Saudi Arabia, fifteen flocks from Eastern region (Al Ahsa, Dammam and Hafr Albaten) and ten flocks from Riyadh and Al-Kharj regions were selected for this study. A total of 700 samples (400 nasal swabs, 300 pleural fluid samples and lung samples (from necropsied animals)) were collected from goats showing typical signs of CCPP. The clinical signs of diseased cases revealed serous to mucoid nasal discharge, coughing, dyspnea, frothy salivation, and fever (40-42°C). Necropsied animals showed fibrinous pleuropneumonia and increased pleural fluid. Of 400 nasal swabs, 190 pleural fluid, and 110 lung samples, 26 (6.5%), 31 (16.3%) and 19 (17.3%) Mycoplasma isolates were recovered, respectively. Biochemically, all isolates were sensitive to digitonin and fermented glucose. Sixty seven of Mycoplasma isolates were belonged to Mycoplasma mycoides cluster based on detection of 16S rRNA. Polymerase chain reaction screening of Mycoplasma isolates using specific primer for M. capricolum subsp. capripneumoniae confirmed 55 isolates to be M. capricolum subsp. capripneumoniae.
经典型山羊传染性胸膜肺炎是世界动物卫生组织列出的最致命的山羊传染病之一，会导致重大经济损失。它由山羊支原体山羊肺炎亚种感染引起。为了首次在沙特阿拉伯王国分离出山羊传染性胸膜肺炎的病原体，本研究选取了东部地区（艾哈萨、达曼和哈费尔巴廷）的15个羊群以及利雅得和哈吉尔地区的10个羊群。从表现出山羊传染性胸膜肺炎典型症状的山羊身上共采集了700份样本（400份鼻拭子、300份胸水样本和肺样本（来自剖检动物））。患病病例的临床症状表现为浆液性至黏液性鼻液、咳嗽、呼吸困难、流泡沫唾液和发热（40 - 42°C）。剖检动物显示出纤维素性胸膜肺炎和胸水增多。在400份鼻拭子、190份胸水样本和110份肺样本中，分别分离出26株（6.5%）、31株（16.3%）和19株（17.3%）支原体。生化分析表明，所有分离株对洋地黄皂苷敏感并能发酵葡萄糖。基于16S rRNA检测，67株支原体分离株属于丝状支原体簇。使用山羊支原体山羊肺炎亚种特异性引物对支原体分离株进行聚合酶链反应筛选，确认55株分离株为山羊支原体山羊肺炎亚种。
