Walz Paul H, Givens M Daniel, Rodning Soren P, Riddell Kay P, Brodersen Bruce W, Scruggs Daniel, Short Thomas, Grotelueschen Dale
College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
Vaccine. 2017 Feb 15;35(7):1046-1054. doi: 10.1016/j.vaccine.2017.01.006. Epub 2017 Jan 19.
The objective of this study was to compare reproductive protection in cattle against bovine viral diarrhea virus (BVDV) and bovine herpesvirus 1 (BoHV-1) provided by annual revaccination with multivalent modified-live viral (MLV) vaccine or multivalent combination viral (CV) vaccine containing temperature-sensitive modified-live BoHV-1 and killed BVDV when MLV vaccines were given pre-breeding to nulliparous heifers. Seventy-five beef heifers were allocated into treatment groups A (n=30; two MLV doses pre-breeding, annual revaccination with MLV vaccine), B (n=30; two MLV doses pre-breeding, annual revaccination with CV vaccine) and C (n=15; saline in lieu of vaccine). Heifers were administered treatments on days 0 (weaning), 183 (pre-breeding), 366 (first gestation), and 738 (second gestation). After first calving, primiparous cows were bred, with pregnancy assessment on day 715. At that time, 24 group A heifers (23 pregnancies), 23 group B heifers (22 pregnancies), and 15 group C heifers (15 pregnancies) were commingled with six persistently infected (PI) cattle for 16days. Ninety-nine days after PI removal, cows were intravenously inoculated with BoHV-1. All fetuses and live offspring were assessed for BVDV and BoHV-1. Abortions occurred in 3/23 group A cows, 1/22 group B cows, and 11/15 group C cows. Fetal infection with BVDV or BoHV-1 occurred in 4/23 group A offspring, 0/22 group B offspring, and 15/15 group C offspring. This research demonstrates efficacy of administering two pre-breeding doses of MLV vaccine with annual revaccination using CV vaccine to prevent fetal loss due to exposure to BVDV and BoHV-1.
本研究的目的是比较在初产小母牛配种前接种多价改良活病毒（MLV）疫苗或含有温度敏感型改良活BoHV-1和灭活BVDV的多价组合病毒（CV）疫苗进行年度再接种时，牛对牛病毒性腹泻病毒（BVDV）和牛疱疹病毒1型（BoHV-1）的生殖保护情况。75头肉用小母牛被分配到治疗组A（n = 30；配种前接种两剂MLV疫苗，每年用MLV疫苗再接种）、B（n = 30；配种前接种两剂MLV疫苗，每年用CV疫苗再接种）和C（n = 15；用生理盐水代替疫苗）。小母牛在第0天（断奶）、183天（配种前）、366天（首次妊娠）和738天（第二次妊娠）接受治疗。首次产犊后，初产母牛进行配种，并在第715天进行妊娠评估。此时，将24头A组小母牛（23头怀孕）、23头B组小母牛（22头怀孕）和15头C组小母牛（15头怀孕）与6头持续感染（PI）牛混合饲养16天。去除PI牛99天后，给母牛静脉接种BoHV-1。对所有胎儿和存活后代进行BVDV和BoHV-1评估。A组23头母牛中有3头流产，B组22头母牛中有1头流产，C组15头母牛中有11头流产。A组23头后代中有4头胎儿感染BVDV或BoHV-1，B组22头后代中无胎儿感染，C组15头后代中有15头胎儿感染。本研究证明，在配种前接种两剂MLV疫苗并每年用CV疫苗再接种可有效预防因接触BVDV和BoHV-1而导致的胎儿损失。
