Borgohain M P, Chowdhury L, Ahmed S, Bolshette N, Devasani K, Das T J, Mohapatra A, Lahkar M
Department of Pharmacology & Toxicology, National Institute of Pharmaceutical Education and Research, Guwahati, Assam, India.
Institute Level Biotech-hub, National Institute of Pharmaceutical Education & Research, Guwahati, Assam, India.
J Ethnopharmacol. 2017 Feb 23;198:451-459. doi: 10.1016/j.jep.2017.01.035. Epub 2017 Jan 19.
Paederia foetida Linn. (Family: Rubiaceae) is widely used as a natural remedy for diabetes mellitus by the Nepali and Lepcha tribes of Sikkim and Darjeeling Himalayan region. The plant is administered to a diabetic individual in the form of leaf infusion for 2-3 weeks. Therefore, we investigated the effects of methanolic leaf extract of Paederia foetida (MEPF) on alloxan (ALX) induced diabetic renal oxidative stress and NF-kB dependent renoinflammatory events in rat. Method Effects of MEPF on blood glucose, glomerular filtration rate (GFR), serum and oxidative stress markers were evaluated in ALX induced diabetic wistar rats. Enzyme linked immunosorbent assay (ELISA) was carried out to estimate serum IL-6, IL-1β, TNF-α and renal tissue NF-kB p65 levels. MEPF treatment was given to the diabetic rats at a dose of 250 and 500mg/kg body weight (b.w.).
RESULTS & DISCUSSION: MEPF treatment significantly reduced hyperglycaemia, serum creatinine, blood urea nitrogen (BUN), bilirubin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides (TRIGs), and total cholesterol (TCHOL) levels in the diabetic rats, whereas it significantly restored GFR and serum albumin level. The activity of enzymatic and non-enzymatic antioxidant machineries was remarkably augmented by MEPF treatment. Likewise, it also significantly lowered lipid peroxidation which was evidenced by decreased concentration of thiobarbituric reactive substances (malondialdehyde, MDA) in the renal tissue of treated diabetic groups. Moreover, MEPF treated animals exhibited low serum concentrations of IL-6, IL-1β and TNF-α compared to the diabetic control rats. It showed a dose dependent inhibition of NF-kB activation in the diabetic kidney, but the effects were more prominent at a dose of 500mg/kg. Histopathological examinations also confirmed its nephroprotective action during diabetes.
MEPF treatment mitigates oxidative stress and suppresses renal inflammation via inhibition of NF-kB in diabetic kidney in early progressive diabetic nephropathy.
鸡矢藤（茜草科）被锡金和大吉岭喜马拉雅地区的尼泊尔和雷布查部落广泛用作糖尿病的天然疗法。该植物以叶浸剂的形式给予糖尿病患者2至3周。因此，我们研究了鸡矢藤甲醇叶提取物（MEPF）对大鼠四氧嘧啶（ALX）诱导的糖尿病肾氧化应激和NF-κB依赖性肾炎症事件的影响。方法 在ALX诱导的糖尿病Wistar大鼠中评估MEPF对血糖、肾小球滤过率（GFR）、血清和氧化应激标志物的影响。采用酶联免疫吸附测定（ELISA）法检测血清IL-6、IL-1β、TNF-α和肾组织NF-κB p65水平。以250和500mg/kg体重（b.w.）的剂量对糖尿病大鼠进行MEPF治疗。
MEPF治疗显著降低了糖尿病大鼠的高血糖、血清肌酐、血尿素氮（BUN）、胆红素、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、甘油三酯（TRIGs）和总胆固醇（TCHOL）水平，同时显著恢复了GFR和血清白蛋白水平。MEPF治疗显著增强了酶促和非酶促抗氧化机制的活性。同样，它还显著降低了脂质过氧化，这在治疗的糖尿病组肾组织中硫代巴比妥酸反应性物质（丙二醛，MDA）浓度降低得到证实。此外，与糖尿病对照大鼠相比，MEPF治疗的动物血清IL-6、IL-1β和TNF-α浓度较低。它显示出对糖尿病肾脏中NF-κB激活的剂量依赖性抑制，但在500mg/kg剂量时效果更显著。组织病理学检查也证实了其在糖尿病期间的肾保护作用。
在早期进行性糖尿病肾病中，MEPF治疗通过抑制糖尿病肾脏中的NF-κB减轻氧化应激并抑制肾炎症。
