School of Food Science and Engineering, Hainan University/Engineering Research Center of Utilization of Tropical polysaccharide resources, Ministry of Education/Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Haikou 570228, China.
Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key laboratory of Agricultural Products Processing, Guangzhou 510610, China.
Ultrason Sonochem. 2022 May;86:106005. doi: 10.1016/j.ultsonch.2022.106005. Epub 2022 Apr 12.
Ultrasonic-assisted extraction (UAE) coupled with deep eutectic solvent (DES) is a novel, efficient and green extraction method for phytochemicals. In this study, the effects of 16 DESs coupled with UAE on the extraction rate of polyphenols from Paederia scandens (Lour.) Merr. (P. scandens), an edible and medicinal herb, were investigated. DES synthesised with choline chloride and ethylene glycol at a 1:2 M ratio resulted in the highest extractability. Moreover, the effects of extraction parameters were investigated by using a two-level factorial experiment followed by response surface methodology The optimal parameters (water content in DES of 49.2%, the actual ultrasonic power of 72.4 W, and ultrasonic time of 9.7 min) resulted in the optimal total flavonoid content (TFC) (27.04 mg CE/g DW), ferric-reducing antioxidant power (FRAP) value (373.27 μmol Fe(Ⅱ)E/g DW) and 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid radical (ABTS) value (48.64 μmol TE/g DW), closely matching the experimental results. Furthermore, a comparison study demonstrated that DES-UAE afforded the higher TFC and FRAP value than traditional extraction methods. 36 individual polyphenolic compounds were identified and quantified by ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) in P. scandens extracts, and of which 30 were found in the extracts obtained by DES-UAE. Additionally, DES-UAE afforded the highest sum of individual polyphenolic compound content. These results revealed that DES-UAE enhanced the extraction efficiency for polyphenols and provided a scientific basis for further processing and utilization of P. scandens.
超声辅助提取 (UAE) 与深共晶溶剂 (DES) 结合是一种新型、高效、绿色的提取植物化学物质的方法。本研究考察了 16 种 DES 与 UAE 结合对食用药用草药鸡矢藤 (Paederia scandens (Lour.) Merr.) 中多酚提取率的影响。以胆碱氯化物和乙二醇摩尔比为 1:2 合成的 DES 具有最高的提取能力。此外，通过两水平因子实验和响应面法考察了提取参数的影响。最佳参数（DES 中水含量为 49.2%，实际超声功率为 72.4 W，超声时间为 9.7 min）得到了最佳总黄酮含量（TFC）（27.04 mg CE/g DW）、铁还原抗氧化能力（FRAP）值（373.27 μmol Fe(Ⅱ)E/g DW）和 2,2'-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)自由基（ABTS）值（48.64 μmol TE/g DW），与实验结果非常吻合。此外，对比研究表明，DES-UAE 比传统提取方法具有更高的 TFC 和 FRAP 值。通过超高效液相色谱-质谱联用仪（UHPLC-MS）在鸡矢藤提取物中鉴定和定量了 36 种单体多酚化合物，其中 30 种在 DES-UAE 提取物中被发现。此外，DES-UAE 提供了最高的单体多酚化合物含量总和。这些结果表明，DES-UAE 提高了多酚的提取效率，为进一步加工和利用鸡矢藤提供了科学依据。
