Carbó Anna, Torres Rosario, Usall Josep, Fons Estanislau, Teixidó Neus
IRTA, XaRTA-Postharvest, Edifici Fruitcentre, Parc Científic i Tecnològic Agroalimentari de Lleida, Lleida, Catalonia, Spain.
Food Technology Department, Lleida University, XaRTA-Postharvest, Agrotecnio Center, Lleida, Catalonia, Spain.
J Sci Food Agric. 2017 Aug;97(11):3691-3698. doi: 10.1002/jsfa.8229. Epub 2017 Feb 23.
The biocontrol agent Candida sake CPA-1 is effective against several diseases. Consequently, the optimisation of a dry formulation of C. sake to improve its shelf life and manipulability is essential for increasing its potential with respect to future commercial applications. The present study aimed to optimise the conditions for making a dry formulation of C. sake using a fluidised bed drying system and then to determine the shelf life of the optimised formulation and its efficacy against Penicillium expansum on apples.
The optimal conditions for the drying process were found to be 40 °C for 45 min and the use of potato starch as the carrier significantly enhanced the viability. However, none of the protective compounds tested increased the viability of the dried cells. A temperature of 25 °C for 10 min in phosphate buffer was considered as the optimum condition to recover the dried formulations. The dried formulations should be stored at 4 °C and air-packaged; moreover, shelf life assays indicated good results after 12 months of storage. The formulated products maintained their biocontrol efficacy.
A fluidised bed drying system is a suitable process for dehydrating C. sake cells; moreover, the C. sake formulation is easy to pack, store and transport, and is a cost-effective process. © 2017 Society of Chemical Industry.
生防菌清酒假丝酵母CPA-1对多种病害有效。因此，优化清酒假丝酵母的干制剂以提高其保质期和可操作性对于提升其在未来商业应用中的潜力至关重要。本研究旨在优化使用流化床干燥系统制备清酒假丝酵母干制剂的条件，然后确定优化制剂的保质期及其对苹果上扩展青霉的防治效果。
发现干燥过程的最佳条件为40℃ 45分钟，使用马铃薯淀粉作为载体可显著提高存活率。然而，所测试的任何保护化合物均未提高干燥细胞的存活率。在磷酸盐缓冲液中25℃ 10分钟的温度被认为是恢复干燥制剂的最佳条件。干燥制剂应在4℃下储存并采用空气包装；此外，保质期试验表明储存12个月后结果良好。制剂产品保持了其生防效果。
流化床干燥系统是清酒假丝酵母细胞脱水的合适工艺；此外，清酒假丝酵母制剂易于包装、储存和运输，且成本效益高。© 2017化学工业协会。
