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[铅污染雏鸡晶状体蛋白的组成]

[Composition of lens protein in chicks contaminated by lead].

作者信息

Gruszczyńska M

出版信息

Klin Oczna. 1989 Jan;91(1):7-8.

PMID:2811193
Abstract

Six days old embryos of domestic hen were contaminated with lead. The 1st group received 10 mg Pb/kg, the 2d - 50 mg Pb/kg and the 3d (control group) distilled water. On the second day after the chicks hatched ophthalmological examinations were performed; the animal was decapitated, the eyes enucleated, lens extracted and homogenized. The composition of the lens proteins was investigated by means of blotting paper and polyacrylamide electrophoresis. The contaminated group showed a higher percentage of a fast-migration--high molecular protein than in the control group.

摘要

将6日龄的家鸡胚胎用铅污染。第一组接受10毫克铅/千克,第二组接受50毫克铅/千克,第三组(对照组)接受蒸馏水。雏鸡孵化后第二天进行眼科检查;将动物断头,摘除眼球,取出晶状体并匀浆。通过滤纸和聚丙烯酰胺电泳研究晶状体蛋白质的组成。污染组中快速迁移的高分子量蛋白质的百分比高于对照组。

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