Fan Wenqiang, Wang Wenxiang, Wu Jie, Ma Ling, Guo Jinyan
Department of Rheumatology, The Central Hospital of Xinxiang, Xinxiang, Henan, China.
Department of Gynecology Oncology, The Central Hospital of Xinxiang, Xinxiang, Henan, China.
Biomark Med. 2017 Feb;11(2):107-116. doi: 10.2217/bmm-2016-0261. Epub 2017 Jan 23.
This study aimed to identify CD4 T-cell-derived microparticles (MPs) and investigate their roles in rheumatoid arthritis (RA).
Synovial fluids from 34 RA, 33 osteoarthritis patients and 42 healthy individuals were analyzed by flow cytometry. Human fibroblast-like synoviocytes and peripheral blood mononuclear cells were cultured with or without isolated MPs, chemokines and cytokines were measured by ELISA.
CD4CD161CD39 and CD4CD39CD73 MPs were abundantly present in RA patients, which were positively or negatively correlated with RA features, respectively. Chemokines CCL20, CCL17 and CCL22, and cytokines IL-17 and IL-10 were influenced by these MPs in human fibroblast-like synoviocytes (HFLS) or PMBCs.
CD4 T-cell-derived CD161CD39 and CD39CD73 MPs could serve as new reciprocal biomarkers for RA evaluation.
本研究旨在鉴定CD4 T细胞衍生的微粒（MPs），并研究它们在类风湿性关节炎（RA）中的作用。
采用流式细胞术分析34例类风湿性关节炎患者、33例骨关节炎患者和42例健康个体的滑液。将人成纤维细胞样滑膜细胞和外周血单核细胞与分离的微粒一起培养或不培养，通过酶联免疫吸附测定法测量趋化因子和细胞因子。
CD4CD161CD39和CD4CD39CD73微粒大量存在于类风湿性关节炎患者中，分别与类风湿性关节炎特征呈正相关或负相关。趋化因子CCL20、CCL17和CCL22以及细胞因子IL-17和IL-10在人成纤维细胞样滑膜细胞（HFLS）或外周血单核细胞中受这些微粒影响。
CD4 T细胞衍生的CD161CD39和CD39CD73微粒可作为评估类风湿性关节炎的新型相互生物标志物。
