Du Chao, Guo Yahui, Cheng Yuliang, Han Mei, Zhang Weiguo, Qian He
a School of Food Science and Technology, Jiangnan University , Wuxi , Jiangsu Province , PR China.
b School of Biotechnology, Jiangnan University , Wuxi , Jiangsu Province , PR China.
Free Radic Res. 2017 Feb;51(2):113-123. doi: 10.1080/10715762.2017.1285024. Epub 2017 Feb 14.
The current study was designed to elucidate the cytoprotective effects and possible mechanisms of torulene and torularhodin on hydrogen peroxide (HO)-induced oxidative stress damage in human prostate stromal cells (WPMY-1). After treated with HO, a notable decrease was appeared in cell viability, yet the decrease was attenuated when cells were pretreated with torulene and torularhodin (0.5-10 μM) as evaluated by WST-1 assay. Pretreatment with these two carotenoids significantly attenuated HO-induced apoptosis in WPMY-1 cells through the inhibition of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) overproduction, as well as the activation of the activities in catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px). Finally, pretreatment of cells with carotenoids resulted in the regulation of the mRNA and protein expression of Bcl-2 and Bax in HO-exposed prostate stromal cells. The present results indicate that both torulene and torularhodin can protect human prostate stromal cells from oxidative stress damage via Bcl-2/Bax mediated apoptosis.
本研究旨在阐明虾青素和虾红素对过氧化氢（H₂O₂）诱导的人前列腺基质细胞（WPMY-1）氧化应激损伤的细胞保护作用及其可能机制。用H₂O₂处理后，细胞活力显著下降，但通过WST-1法评估发现，当细胞用虾青素和虾红素（0.5-10μM）预处理时，这种下降得到缓解。这两种类胡萝卜素预处理通过抑制细胞内活性氧（ROS）和丙二醛（MDA）的过量产生，以及激活过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的活性，显著减轻了H₂O₂诱导的WPMY-1细胞凋亡。最后，用类胡萝卜素预处理细胞导致了H₂O₂处理的前列腺基质细胞中Bcl-2和Bax的mRNA和蛋白表达的调节。目前的结果表明，虾青素和虾红素均可通过Bcl-2/Bax介导的凋亡保护人前列腺基质细胞免受氧化应激损伤。
