Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo, Spain.
Departamento de Química Física and CINBIO, Universidade de Vigo, 36310 Vigo, Spain.
Nanoscale. 2017 Feb 2;9(5):2051-2058. doi: 10.1039/c6nr08432j.
Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point of care (POC) test option, but they have some limitations in terms of detection and quantification. In this work we have developed a lateral flow immunoassay for the ultrasensitive detection of penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labelled probe. The combination of Au@Ag core-shell nanoparticles as plasmonic platform and Rhodamine B Isothiocyanate as Raman reporter has allowed us to fabricate a SERRS tag with high efficiency and reliability. The limit of detection of the SERRS-based LFIA was 1 pg mL. This could be a strong foundation for a pneumonia diagnosis test based on pneumolysin detection.
使用传统检测方法明确诊断肺炎较为困难且费用较高。侧向流动免疫分析（LFIAs）是一种有优势的即时检测（POC）试验选择，但在检测和定量方面存在一些局限性。在这项工作中，我们开发了一种侧向流动免疫分析方法，用于超灵敏检测肺炎球菌溶血素，采用等离子体表面增强共振拉曼散射（SERRS）标记作为标记探针。金-银核壳纳米粒子作为等离子体平台和罗丹明 B 异硫氰酸酯作为拉曼报告基团的组合，使我们能够制备具有高效和可靠性的 SERRS 标记物。基于 SERRS 的 LFIA 的检测限为 1 pg mL-1。这可能为基于肺炎球菌溶血素检测的肺炎诊断测试奠定坚实的基础。
