Fu Yu-Fang, Zhu Jian
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
Mikrochim Acta. 2025 Jul 12;192(8):501. doi: 10.1007/s00604-025-07352-1.
Gold nanoparticles (AuNPs) are widely used in biomedical sensing due to their tunable localized surface plasmon resonance (LSPR) optical properties. The need for traditional sensing methods that do not offer the advantages of timeliness and portability has been addressed by the introduction of lateral flow immunoassay (LFIA) strips, a point-of-care testing (POCT) tool, which offers the advantages of simplicity of handling, visualization, and short detection times. Thus, the combination between LSPR of AuNPs and LFIA strips has evolved to provide a powerful platform capable of ultrasensitive and multiplexed detection of a wide range of target analytes. In recent years, many new types of AuNPs with special morphologic structure and excellent plasmonic optical properties have been developed and used in LFIA. This review focuses on recent advances in novel morphologies and modifications of AuNPs as probes for LFIA strips, highlighting their applications in POCT detection through colorimetric, surface-enhanced Raman scattering, fluorescent, photothermal, and multimodal approaches.
金纳米颗粒（AuNPs）因其可调谐的局域表面等离子体共振（LSPR）光学性质而被广泛应用于生物医学传感。即时检测（POCT）工具——侧向流动免疫分析（LFIA）条带的引入，解决了传统传感方法缺乏及时性和便携性的问题，该工具具有操作简单、可视化和检测时间短等优点。因此，AuNPs的LSPR与LFIA条带的结合已发展成为一个强大的平台，能够对多种目标分析物进行超灵敏和多重检测。近年来，许多具有特殊形态结构和优异等离子体光学性质的新型AuNPs已被开发并应用于LFIA。本文综述了作为LFIA条带探针的新型AuNPs形态和修饰的最新进展，重点介绍了它们在POCT检测中的比色、表面增强拉曼散射、荧光、光热和多模态方法的应用。
